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PMID: 9465098 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Evidence that the lipid moiety of oxidized low density lipoprotein plays a role in its interaction with macrophage receptors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 4 ·1998-02-17 ·Pages 1806-11

Terpstra V, Bird DA, Steinberg D

Abstract

The binding of oxidatively damaged red blood cells (OxRBCs) to resident mouse peritoneal macrophages correlates with an increase in phosphatidylserine on the external leaflet of the plasma membrane. Liposomes rich in phosphatidylserine can inhibit this binding and also the binding of certain apoptotic cells. We have shown previously that oxidized low density lipoproteins (OxLDL) also can inhibit the binding of OxRBCs to resident mouse peritoneal macrophages. The present studies show that microemulsions prepared from the lipids extracted from OxLDL are very effective in inhibiting the binding of OxRBCs and also, to a lesser extent, of apoptotic thymocytes to macrophages. OxRBC binding was also inhibited by cholesterol phospholipid liposomes containing oxidized 1-stearoyl-2-linoleoyl-phosphatidylcholine. The binding and uptake of 125I-labeled OxLDL were also strongly inhibited by microemulsions of the lipids extracted from OxLDL and by cholesterol phospholipid liposomes containing oxidized 1-palmitoyl-2-arachidonoyl-phosphatidylcholine. Earlier studies have shown that the delipidated protein moiety of OxLDL can competitively inhibit macrophage binding of intact OxLDL, implicating the protein moiety as an effective receptor-binding domain of OxLDL with respect to some macrophage scavenger receptors. The present studies suggest that the lipid moiety of OxLDL may also play a role.

MeSH Terms
Animals Apolipoproteins B/metabolism Emulsions Humans Lipid Metabolism Lipoproteins, LDL/metabolism Liposomes Macrophages, Peritoneal/metabolism Membrane Proteins Mice Phagocytosis Receptors, Immunologic/metabolism Receptors, Lipoprotein Receptors, Scavenger Scavenger Receptors, Class B Structure-Activity Relationship
Chemicals
Apolipoproteins B Emulsions Lipoproteins, LDL Liposomes Membrane Proteins Receptors, Immunologic Receptors, Lipoprotein Receptors, Scavenger Scarb1 protein, mouse Scavenger Receptors, Class B acetyl-LDL oxidized low density lipoprotein
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Terpstra V
Department of Medicine 0682, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0682, USA.
Bird D A
Steinberg D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.4.1806
Published
1998-02-17
Pages
1806-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC19194
Subset
IM
Grants
NHLBI NIH HHS · HL-14197 · United States
External Links
PubMed source DOI: 10.1073/pnas.95.4.1806 PMC Full Text View journal details
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