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PMID: 9482928 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A substrate of the centisome 63 type III protein secretion system of Salmonella typhimurium is encoded by a cryptic bacteriophage.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 5 ·1998-03-03 ·Pages 2574-9

Hardt WD, Urlaub H, Galán JE

Abstract

Salmonella enterica has evolved a type III protein secretion system that allows these enteropathogens to translocate effector molecules directly into the host cell cytoplasm. These effectors mediate a variety of responses, including cytoskeletal rearrangements, cytokine production, and in certain cells, the induction of apoptosis. We report here the characterization of a substrate of this secretion system in S. enterica serovar typhimurium (Salmonella typhimurium) that is homologous to the SopE protein of Salmonella dublin implicated in bacterial entry into cultured epithelial cells. The sopE locus is located within a cluster of genes that encode tail and tail fiber proteins of a cryptic P2-like prophage, outside of the centisome 63 pathogenicity island that encodes the invasion-associated type III secretion system. Southern hybridization analysis revealed that sopE is present in only a subset of S. enterica serovars and that the flanking bacteriophage genes are also highly polymorphic. Encoding effector proteins that are delivered through type III secretion systems in highly mobile genetic elements may allow pathogens to adapt rapidly by facilitating the assembly of an appropriate set of effector proteins required for successful replication in a new environment.

MeSH Terms
Amino Acid Sequence Animals Bacterial Proteins/biosynthesis,chemistry,genetics Genes, Viral Genotype Mice Molecular Sequence Data Multigene Family Salmonella/genetics Salmonella Infections, Animal/physiopathology Salmonella Phages/genetics,physiology Salmonella typhimurium/genetics,pathogenicity,virology Sequence Alignment Substrate Specificity Viral Proteins/biosynthesis,chemistry,genetics Virulence
Chemicals
Bacterial Proteins SopE protein, Salmonella Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hardt W D
Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook, Stony Brook, NY 11794-5222, USA.
Urlaub H
Galán J E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.5.2574
Published
1998-03-03
Pages
2574-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC19418
Subset
IM
Grants
NIAID NIH HHS · R01 AI030492 · United States
NIAID NIH HHS · R37 AI030492 · United States
NIAID NIH HHS · AI30492 · United States
NIGMS NIH HHS · GM52543 · United States
Databases
GENBANK
AF043239
External Links
PubMed source DOI: 10.1073/pnas.95.5.2574 PMC Full Text View journal details
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