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PMID: 9495766 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The spoIIE locus is involved in the Spo0A-dependent switch in the location of FtsZ rings in Bacillus subtilis.

Journal of bacteriology ·Vol. 180 ·No. 5 ·1998-03-00 ·Pages 1256-60

Khvorova A, Zhang L, Higgins ML, Piggot PJ

Abstract

A switch in the location of FtsZ ring structures from medial to polar is one of the earliest morphological indicators of sporulation in Bacillus subtilis. This switch can be artificially caused during vegetative growth by induction of an active form, Sad67, of the transcription regulator, Spo0A (P. A. Levin and R. Losick, Genes Dev. 10:478-488, 1996). We have used immunofluorescence microscopy to show that the switch in FtsZ ring location during vegetative growth caused by Sad67 induction is blocked by a spoIIE deletion mutation. The spoIIE mutation also impaired polar FtsZ ring formation during sporulation. These results suggest that SpoIIE mediates the Spo0A-directed formation of polar FtsZ rings.

MeSH Terms
Bacillus subtilis/chemistry,genetics,growth & development,physiology Bacterial Proteins/analysis,genetics,physiology Culture Media Cytoskeletal Proteins Gene Expression Regulation, Bacterial Genes, Bacterial Microscopy, Fluorescence Sequence Deletion Sigma Factor Spores, Bacterial/physiology Transcription Factors/physiology
Chemicals
Bacterial Proteins Culture Media Cytoskeletal Proteins FtsZ protein, Bacteria Sigma Factor Spo0A protein, Bacillus subtilis Transcription Factors spoIIR protein, Bacillus subtilis spore-specific proteins, Bacillus
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Khvorova A
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
Zhang L
Higgins M L
Piggot P J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.5.1256-1260.1998
Published
1998-03-00
Pages
1256-60
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC107015
Subset
IM
Grants
NIGMS NIH HHS · R01 GM043577 · United States
NIGMS NIH HHS · GM-43577 · United States
NIGMS NIH HHS · GM-51335 · United States
External Links
PubMed source DOI: 10.1128/JB.180.5.1256-1260.1998 PMC Full Text View journal details
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