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PMID: 9502812 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Astrocytic gap junctions remain open during ischemic conditions.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 18 ·No. 7 ·1998-04-01 ·Pages 2520-37

Cotrina ML, Kang J, Lin JH, Bueno E, Hansen TW, He L, Liu Y, Nedergaard M

Abstract

Gap junctions are highly conductive channels that allow the direct transfer of intracellular messengers such as Ca2+ and inositol triphosphate (IP3) between interconnected cells. In brain, astrocytes are coupled extensively by gap junctions. We found here that gap junctions among astrocytes in acutely prepared brain slices as well as in culture remained open during ischemic conditions. Uncoupling first occurred after the terminal loss of plasma membrane integrity. Gap junctions therefore may link ischemic astrocytes in an evolving infarct with the surroundings. The free exchange of intracellular messengers between dying and potentially viable astrocytes might contribute to secondary expansion of ischemic lesions.

MeSH Terms
Animals Apoptosis/physiology Astrocytes/cytology,physiology Brain Ischemia/metabolism Calcium/metabolism,pharmacology Cell Membrane/metabolism Cell Survival/physiology Cells, Cultured Cerebral Cortex/blood supply,cytology Cerebral Infarction/metabolism Enzyme Inhibitors/pharmacology Female Fluorescent Dyes/pharmacokinetics Gap Junctions/drug effects,immunology,metabolism Hippocampus/blood supply,cytology Hydrogen-Ion Concentration Ionophores/pharmacology Isoquinolines/pharmacokinetics Male Organ Culture Techniques Phosphorylation Protons Rats Rats, Sprague-Dawley Second Messenger Systems/physiology Thapsigargin/pharmacology
Chemicals
Enzyme Inhibitors Fluorescent Dyes Ionophores Isoquinolines Protons Thapsigargin lucifer yellow Calcium
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Cotrina M L
Department of Cell Biology, New York Medical College, Valhalla, New York 10595, USA.
Kang J
Lin J H
Bueno E
Hansen T W
He L
Liu Y
Nedergaard M
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1998-04-01
Pages
2520-37
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6793088
Subset
IM
Grants
NINDS NIH HHS · NS130007 · United States
NINDS NIH HHS · NS135011 · United States
External Links
PubMed source PMC Full Text View journal details
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