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PMID: 9512521 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cell cycle-dependent transcriptional and proteolytic regulation of FtsZ in Caulobacter.

Genes & development ·Vol. 12 ·No. 6 ·1998-03-15 ·Pages 880-93

Kelly AJ, Sackett MJ, Din N, Quardokus E, Brun YV

Abstract

In the differentiating bacterium Caulobacter crescentus, the cell division initiation protein FtsZ is present in only one of the two cell types. Stalked cells initiate a new round of DNA replication immediately after cell division and contain FtsZ, whereas the progeny swarmer cells are unable to initiate DNA replication and do not contain FtsZ. We show that FtsZ expression is controlled by cell cycle-dependent transcription and proteolysis. Transcription of ftsZ is repressed in swarmer cells and is activated concurrently with the initiation of DNA replication. At the end of the DNA replication period, transcription of ftsZ decreases substantially. We show that the global cell cycle regulator CtrA is involved in the cell cycle control of ftsZ transcription. CtrA binds to a site that overlaps the ftsZ transcription start site. Removal of the CtrA-binding site results in transcription of the ftsZ promoter in swarmer cells. Decreasing the cellular concentration of CtrA increases ftsZ transcription and conversely, increasing the concentration of CtrA decreases ftsZ transcription. Because CtrA is present in swarmer cells, is degraded at the same time as ftsZ transcription begins, and reappears when ftsZ transcription decreases at the end of the cell cycle, we propose that CtrA is a repressor of ftsZ transcription. We show that proteolysis is an important determinant of cell type-specific distribution and cell cycle variation of FtsZ. FtsZ is stable when it is synthesized and assembles into the cytokinetic ring at the beginning of the cell cycle. After the initiation of cell division, the rate of FtsZ degradation increases as both the constriction site and the FtsZ ring decrease in diameter. When ftsZ is expressed constitutively from inducible promoters, the abundance of FtsZ still varies during the cell cycle. The coupling of transcription and proteolysis to cell division ensures that FtsZ is inherited only by the progeny cell that will begin DNA replication immediately after cell division.

MeSH Terms
Bacterial Proteins/genetics,physiology Base Sequence Binding Sites/genetics Caulobacter crescentus/chemistry,genetics,physiology Cell Cycle/genetics,physiology Cell Division/genetics,physiology Cytoskeletal Proteins DNA-Binding Proteins GTP-Binding Proteins/genetics,physiology Gene Expression Regulation, Bacterial Hydrolysis Molecular Sequence Data Mutation/genetics,physiology Promoter Regions, Genetic/genetics Protein Processing, Post-Translational Sequence Homology, Nucleic Acid Time Factors Transcription Factors Transcription, Genetic/genetics,physiology
Chemicals
Bacterial Proteins CtrA protein, Caulobacter Cytoskeletal Proteins DNA-Binding Proteins FtsZ protein, Bacteria Transcription Factors GTP-Binding Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kelly A J
Department of Biology, Indiana University, Bloomington, Indiana 47405, USA.
Sackett M J
Din N
Quardokus E
Brun Y V
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.6.880
Published
1998-03-15
Pages
880-93
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316630
Subset
IM
Grants
NIGMS NIH HHS · R01 GM051986 · United States
NIGMS NIH HHS · GM07757 · United States
NIGMS NIH HHS · GM51986 · United States
External Links
PubMed source DOI: 10.1101/gad.12.6.880 PMC Full Text View journal details
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