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PMID: 9525857 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Induction of lens differentiation by activation of a bZIP transcription factor, L-Maf.

Science (New York, N.Y.) ·Vol. 280 ·No. 5360 ·1998-04-03 ·Pages 115-8

Ogino H, Yasuda K

Abstract

After the vertebrate lens is induced from head ectoderm, lens-specific genes are expressed. Transcriptional regulation of the lens-specific alphaA-crystallin gene is controlled by an enhancer element, alphaCE2. A gene encoding an alphaCE2-binding protein, L-maf(lens-specific maf), was isolated. L-maf expression is initiated in the lens placode and is restricted to lens cells. The gene product L-Maf regulates the expression of multiple genes expressed in the lens, and ectopic expression of this transcription factor converts chick embryonic ectodermal cells and cultured cells into lens fibers. Thus, vertebrate lens induction and differentiation can be triggered by the activation of L-Maf.

MeSH Terms

Amino Acid Sequence Animals Basic-Leucine Zipper Transcription Factors Cell Differentiation Cells, Cultured Chick Embryo Crystallins/genetics DNA, Complementary DNA-Binding Proteins/chemistry,genetics Ectoderm Enhancer Elements, Genetic Eye Proteins/genetics G-Box Binding Factors Gene Expression Regulation, Developmental Genes, Reporter Intermediate Filament Proteins/genetics Lens, Crystalline/cytology,embryology,metabolism Maf Transcription Factors Molecular Sequence Data Promoter Regions, Genetic Recombinant Fusion Proteins/metabolism Transcription Factors/chemistry,genetics,metabolism Transcription, Genetic Transcriptional Activation Transfection

Chemicals

Basic-Leucine Zipper Transcription Factors Crystallins DNA, Complementary DNA-Binding Proteins Eye Proteins G-Box Binding Factors Intermediate Filament Proteins L-MAF protein, Gallus gallus Maf Transcription Factors Recombinant Fusion Proteins Transcription Factors filensin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ogino H

Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan.

Yasuda K

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.280.5360.115

Published

1998-04-03

Pages

115-8

Language

English

Region

United States

NLM ID

0404511

Subset

Databases

GENBANK

AF034570

External Links

PubMed source DOI: 10.1126/science.280.5360.115 View journal details

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Induction of lens differentiation by activation of a bZIP transcription factor, L-Maf.

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