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PMID: 9526013 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Electrical properties of frog saccular hair cells: distortion by enzymatic dissociation.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 18 ·No. 8 ·1998-04-15 ·Pages 2962-73

Armstrong CE, Roberts WM

Abstract

Although it is widely accepted that the electrical resonance seen in many types of auditory and vestibular hair cells contributes to frequency selectivity in these sensory systems, unexplained discrepancies in the frequency (f) and sharpness (Q) of tuning have raised serious questions. For example, enzymatically dissociated hair cells from bullfrog (Rana catesbeiana) sacculus resonate at frequencies well above the range of auditory and seismic stimuli to which the sacculus is most responsive. Such disparities, in addition to others, have led to the proposal that electrical resonance alone cannot account for frequency tuning. Using grassfrog (Rana pipiens) saccular hair cells, we show that the reported discrepancies in f and Q in this organ can be explained by the deleterious effects of enzyme (papain) exposure during cell dissociation. In patch-clamp studies of hair cells in a semi-intact epithelial preparation, we observed a variety of voltage behaviors with frequencies of 35-75 Hz. This range is well below the range of resonant frequencies observed in enzymatically dissociated hair cells and more in tune with the frequency range of natural stimuli to which the sacculus is maximally responsive. The sharpness of tuning also agreed with previous studies using natural stimuli. In contrast to results from enzymatically dissociated hair cells, both a calcium-activated K+ (KCa) current and a voltage-dependent K+ (KV) current contributed to the oscillatory responses of hair cells in the semi-intact preparation. The properties of the KCa and the Ca2+ current were altered by enzymatic dissociation. KV and a small-conductance calcium-activated K+ current were apparently eliminated.

MeSH Terms
4-Aminopyridine/pharmacology Animals Artifacts Calcium/metabolism Cesium Electric Conductivity Hair Cells, Vestibular/physiology,ultrastructure Membrane Potentials/drug effects,physiology Microscopy, Electron, Scanning Papain Patch-Clamp Techniques/standards Peptides/pharmacology Periodicity Potassium/metabolism Rana catesbeiana Saccule and Utricle/cytology Tetraethylammonium/pharmacology Toxins, Biological/pharmacology
Chemicals
Peptides Toxins, Biological Cesium Tetraethylammonium iberiotoxin 4-Aminopyridine Papain Potassium Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Armstrong C E
Institute of Neuroscience, University of Oregon, Eugene, Oregon 97403-1254, USA.
Roberts W M
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1998-04-15
Pages
2962-73
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6792591
Subset
IM
Grants
NIGMS NIH HHS · GM07257 · United States
NINDS NIH HHS · NS27142 · United States
External Links
PubMed source PMC Full Text View journal details
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