Home LiteratureArticle Details

PMID: 9526659 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Development and applications of enhanced green fluorescent protein mutants.

BioTechniques ·Vol. 24 ·No. 3 ·1998-03-00 ·Pages 462-6, 468-71

Stauber RH, Horie K, Carney P, Hudson EA, Tarasova NI, Gaitanaris GA, Pavlakis GN

Abstract

The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their use as an efficient dual-color tagging system and their independent detection in living cells.

MeSH Terms

3T3 Cells Amino Acid Substitution/genetics Animals Biomarkers Flow Cytometry/instrumentation,methods Gene Expression Regulation Genes, Reporter Green Fluorescent Proteins Luminescent Proteins/biosynthesis,genetics Mice Mutation/genetics Recombinant Fusion Proteins/biosynthesis,genetics Spectrometry, Fluorescence Transfection

Chemicals

Biomarkers Luminescent Proteins Recombinant Fusion Proteins Green Fluorescent Proteins

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Stauber R H

National Cancer Institute-FCRDC, Frederick, MD, USA.

Horie K

Carney P

Hudson E A

Tarasova N I

Gaitanaris G A

Pavlakis G N

Article Info

Journal

BioTechniques

Abbr.

Biotechniques

ISSN

0736-6205

DOI

10.2144/98243rr01

Published

1998-03-00

Pages

462-6, 468-71

Language

English

Region

England

NLM ID

8306785

Subset

External Links

PubMed source DOI: 10.2144/98243rr01 View journal details

Analysis Services

Development and applications of enhanced green fluorescent protein mutants.

Contact

WeChat Official Account