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PMID: 9528847 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Vascular endothelial growth factor (VEGF) mRNA isoform expression pattern is correlated with liver metastasis and poor prognosis in colon cancer.

British journal of cancer ·Vol. 77 ·No. 6 ·1998-03-00 ·Pages 998-1002

Tokunaga T, Oshika Y, Abe Y, Ozeki Y, Sadahiro S, Kijima H, Tsuchida T, Yamazaki H, Ueyama Y, Tamaoki N, Nakamura M

Abstract

Vascular endothelial growth factor (VEGF) is a well known factor that induces angiogenesis. Four isoforms, i.e. VEGF206, 189, 165, and 121, have been identified. We examined the isoform patterns of VEGF mRNA using reverse transcription polymerase chain reaction (RT-PCR) analysis in 61 colon cancers. All the colon cancers examined expressed VEGF121. The isoform patterns were classified into three groups: type 1, VEGF121; type 2, VEGF121 + VEGF165; type 3, VEGF121 + VEGF165 + VEGF189. Three of the 61 colon cancers examined showed type 1 expression, 26 showed type 2 expression and 32 showed the type 3 pattern. The patients with liver metastases showed the type 3 isoform expression pattern at a significantly higher incidence (12 of 16, 75%) than those without liver metastasis (20 of 45, 44%) (P=0.036). The type 3 isoform pattern was significantly associated with M1 stage (P=0.019). The patients with colon cancer and the type 3 isoform pattern showed significantly poor prognosis (P < 0.01, Cox-Mantel). The colon cancers with the type 3 pattern showed a significantly higher involvement of veins (P=0.006). These observations suggest that the aberrant type 3 expression pattern of VEGF189 mRNA isoforms is correlated with liver metastasis, M stage, and poor prognosis in colon cancer.

MeSH Terms
Adenocarcinoma/mortality,pathology,secondary,surgery Adenocarcinoma, Mucinous/mortality,pathology,secondary,surgery Colonic Neoplasms/metabolism,mortality,pathology,surgery Endothelial Growth Factors/biosynthesis Female Genes, ras Humans Liver Neoplasms/metabolism,pathology,secondary,surgery Lymphokines/biosynthesis Male Middle Aged Mutation Neoplasm Staging Polymerase Chain Reaction Predictive Value of Tests Prognosis Proto-Oncogene Proteins/biosynthesis RNA, Messenger/biosynthesis Receptor Protein-Tyrosine Kinases/biosynthesis Receptors, Growth Factor/biosynthesis Receptors, Vascular Endothelial Growth Factor Retrospective Studies Survival Rate Time Factors Transcription, Genetic Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-1 Vascular Endothelial Growth Factors
Chemicals
Endothelial Growth Factors Lymphokines Proto-Oncogene Proteins RNA, Messenger Receptors, Growth Factor VEGFA protein, human Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors Receptor Protein-Tyrosine Kinases Receptors, Vascular Endothelial Growth Factor Vascular Endothelial Growth Factor Receptor-1
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Tokunaga T
Department of Pathology, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
Oshika Y
Abe Y
Ozeki Y
Sadahiro S
Kijima H
Tsuchida T
Yamazaki H
Ueyama Y
Tamaoki N
Nakamura M
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Article Info
Journal
British journal of cancer
Abbr.
Br J Cancer
ISSN
0007-0920
DOI
10.1038/bjc.1998.164
Published
1998-03-00
Pages
998-1002
Language
English
Region
England
NLM ID
0370635
PMCID
PMC2150098
Subset
IM
External Links
PubMed source DOI: 10.1038/bjc.1998.164 PMC Full Text View journal details
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