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PMID: 9529095 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Local chemokine paralysis, a novel pathogenic mechanism for Porphyromonas gingivalis.

Infection and immunity ·Vol. 66 ·No. 4 ·1998-04-00 ·Pages 1660-5

Darveau RP, Belton CM, Reife RA, Lamont RJ

Abstract

Periodontitis, which is widespread in the adult population, is a persistent bacterial infection associated with Porphyromonas gingivalis. Gingival epithelial cells are among the first cells encountered by both P. gingivalis and commensal oral bacteria. The chemokine interleukin 8 (IL-8), a potent chemoattractant and activator of polymorphonuclear leukocytes, was secreted by gingival epithelial cells in response to components of the normal oral flora. In contrast, P. gingivalis was found to strongly inhibit IL-8 accumulation from gingival epithelial cells. Inhibition was associated with a decrease in mRNA for IL-8. Antagonism of IL-8 accumulation did not occur in KB cells, an epithelial cell line that does not support high levels of intracellular invasion by P. gingivalis. Furthermore, a noninvasive mutant of P. gingivalis was unable to antagonize IL-8 accumulation. Invasion-dependent destruction of the gingival IL-8 chemokine gradient at sites of P. gingivalis colonization (local chemokine paralysis) will severely impair mucosal defense and represents a novel mechanism for bacterial colonization of host tissue.

MeSH Terms
Dental Plaque/microbiology Humans Interleukin-8/antagonists & inhibitors,genetics,metabolism KB Cells Periodontal Diseases/etiology Porphyromonas gingivalis/pathogenicity RNA, Messenger/analysis
Chemicals
Interleukin-8 RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Darveau R P
Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 98121, USA. [email protected]
Belton C M
Reife R A
Lamont R J
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.66.4.1660-1665.1998
Email
[email protected]
Published
1998-04-00
Pages
1660-5
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC108102
Subset
IM
Grants
NIDCR NIH HHS · R01 DE011111 · United States
NIDCR NIH HHS · R37 DE011111 · United States
NIDCR NIH HHS · DE11111 · United States
External Links
PubMed source DOI: 10.1128/IAI.66.4.1660-1665.1998 PMC Full Text View journal details
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