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PMID: 9537401 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Proton gradient-driven nickel uptake by vacuolar membrane vesicles of Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 180 ·No. 7 ·1998-04-00 ·Pages 1962-4

Nishimura K, Igarashi K, Kakinuma Y

Abstract

A vacuolar H+-ATPase-negative mutant of Saccharomyces cerevisiae was highly sensitive to nickel ion. Accumulation of nickel ion in the cells of this mutant of less than 60% of the value for the parent strain arrested growth, suggesting a role for this ATPase in sequestering nickel ion into vacuoles. An artificially imposed pH gradient (interior acid) induced transient nickel ion uptake by vacuolar membrane vesicles, which was inhibited by collapse of the pH difference but not of the membrane potential. Nickel ion transport into vacuoles in a pH gradient-dependent manner is thus important for its detoxification in yeast.

MeSH Terms
Hydrogen-Ion Concentration Magnesium/pharmacology Nickel/metabolism,pharmacology Proton-Translocating ATPases/physiology Saccharomyces cerevisiae/drug effects,metabolism Vacuoles/metabolism
Chemicals
Nickel Proton-Translocating ATPases Magnesium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nishimura K
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Igarashi K
Kakinuma Y
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20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.7.1962-1964.1998
Published
1998-04-00
Pages
1962-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC107116
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.180.7.1962-1964.1998 PMC Full Text View journal details
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