Home LiteratureArticle Details
PMID: 9539443 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Suppressors of an Arabidopsis thaliana phyB mutation identify genes that control light signaling and hypocotyl elongation.

Genetics ·Vol. 148 ·No. 3 ·1998-03-00 ·Pages 1295-310

Reed JW, Elumalai RP, Chory J

Abstract

Ambient light controls the development and physiology of plants. The Arabidopsis thaliana photoreceptor phytochrome B (PHYB) regulates developmental light responses at both seedling and adult stages. To identify genes that mediate control of development by light, we screened for suppressors of the long hypocotyl phenotype caused by a phyB mutation. Genetic analyses show that the shy (short hypocotyl) mutations we have isolated fall in several loci. Phenotypes of the mutants suggest that some of the genes identified have functions in control of light responses. Other loci specifically affect cell elongation or expansion.

MeSH Terms
Arabidopsis/genetics,physiology Arabidopsis Proteins Darkness Genes, Plant Hypocotyl Light Mutagenesis Phenotype Photoreceptor Cells Phytochrome/genetics Phytochrome B Plant Proteins/genetics Plant Roots/physiology Time Factors Transcription Factors
Chemicals
Arabidopsis Proteins PHYB protein, Arabidopsis Plant Proteins Transcription Factors Phytochrome Phytochrome B
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Reed J W
Biology Department, University of North Carolina, Chapel Hill 27599-3280, USA. [email protected]
Elumalai R P
Chory J
References (56)
56 references, click to expand
  1. A Role for Cytokinins in De-Etiolation in Arabidopsis (det Mutants Have an Altered Response to Cytokinins).
    Plant Physiol. 1994 Feb;104(2):339-347 PMID: 12232085
  2. RED1 is necessary for phytochrome B-mediated red light-specific signal transduction in Arabidopsis.
    Plant Cell. 1997 May;9(5):731-43 PMID: 9165750
  3. Flowering responses to altered expression of phytochrome in mutants and transgenic lines of Arabidopsis thaliana (L.) Heynh.
    Plant Physiol. 1995 Aug;108(4):1495-503 PMID: 7659750
  4. The blue-light receptor cryptochrome 1 shows functional dependence on phytochrome A or phytochrome B in Arabidopsis thaliana.
    Plant J. 1997 Mar;11(3):421-7 PMID: 9107032
  5. The Arabidopsis HY5 gene encodes a bZIP protein that regulates stimulus-induced development of root and hypocotyl.
    Genes Dev. 1997 Nov 15;11(22):2983-95 PMID: 9367981
  6. The phytochrome apoprotein family in Arabidopsis is encoded by five genes: the sequences and expression of PHYD and PHYE.
    Plant Mol Biol. 1994 Jun;25(3):413-27 PMID: 8049367
  7. Conditional root expansion mutants of Arabidopsis.
    Development. 1995 Apr;121(4):1237-52 PMID: 7743935
  8. Nuclear localization activity of phytochrome B.
    Plant J. 1996 Nov;10(5):859-68 PMID: 8953247
  9. Evidence for FUS6 as a component of the nuclear-localized COP9 complex in Arabidopsis.
    Plant Cell. 1996 Nov;8(11):2047-56 PMID: 8953769
  10. Suppressors of trp1 fluorescence identify a new arabidopsis gene, TRP4, encoding the anthranilate synthase beta subunit.
    Plant Cell. 1993 Sep;5(9):1011-27 PMID: 8400875
  11. A chlorate-resistant mutant defective in the regulation of nitrate reductase gene expression in Arabidopsis defines a new HY locus.
    Plant Cell. 1997 Jan;9(1):21-35 PMID: 9014362
  12. A deletion in the PHYD gene of the Arabidopsis Wassilewskija ecotype defines a role for phytochrome D in red/far-red light sensing.
    Plant Cell. 1997 Aug;9(8):1317-26 PMID: 9286109
  13. Novel phytochrome sequences in Arabidopsis thaliana: structure, evolution, and differential expression of a plant regulatory photoreceptor family.
    Genes Dev. 1989 Nov;3(11):1745-57 PMID: 2606345
  14. Phytochrome A null mutants of Arabidopsis display a wild-type phenotype in white light.
    Plant Cell. 1993 Jul;5(7):757-68 PMID: 8364355
  15. Isolation and preliminary characterization of gas1-1, a mutation causing partial suppression of the phenotype conferred by the gibberellin-insensitive (gai) mutation in Arabidopsis thaliana (L.) Heyhn.
    Planta. 1995;197(2):414-7 PMID: 8547820
  16. fhy1 defines a branch point in phytochrome A signal transduction pathways for gene expression.
    Plant J. 1996 Dec;10(6):1155-61 PMID: 9011096
  17. Procuste1 mutants identify two distinct genetic pathways controlling hypocotyl cell elongation, respectively in dark- and light-grown Arabidopsis seedlings.
    Development. 1996 Feb;122(2):683-93 PMID: 8625819
  18. The COP9 complex, a novel multisubunit nuclear regulator involved in light control of a plant developmental switch.
    Cell. 1996 Jul 12;86(1):115-21 PMID: 8689678
  19. Regulatory hierarchy of photomorphogenic loci: allele-specific and light-dependent interaction between the HY5 and COP1 loci.
    Plant Cell. 1994 May;6(5):613-28 PMID: 8038602
  20. Assignment of 30 microsatellite loci to the linkage map of Arabidopsis.
    Genomics. 1994 Jan 1;19(1):137-44 PMID: 8188214
  21. Phytochrome A Mediates the Promotion of Seed Germination by Very Low Fluences of Light and Canopy Shade Light in Arabidopsis.
    Plant Physiol. 1996 Feb;110(2):439-444 PMID: 12226195
  22. Arabidopsis mutants define downstream branches in the phototransduction pathway.
    Genes Dev. 1994 Feb 1;8(3):339-49 PMID: 8314087
  23. LIGHT CONTROL OF SEEDLING DEVELOPMENT.
    Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:215-243 PMID: 15012288
  24. The Induction of Seed Germination in Arabidopsis thaliana Is Regulated Principally by Phytochrome B and Secondarily by Phytochrome A.
    Plant Physiol. 1994 Feb;104(2):363-371 PMID: 12232088
  25. Phenotypic and Genetic Analysis of det2, a New Mutant That Affects Light-Regulated Seedling Development in Arabidopsis.
    Plant Cell. 1991 May;3(5):445-459 PMID: 12324600
  26. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers.
    Plant J. 1993 Aug;4(2):403-10 PMID: 8106085
  27. Phytochrome signal transduction pathways are regulated by reciprocal control mechanisms.
    Genes Dev. 1994 Sep 15;8(18):2188-202 PMID: 7958888
  28. Arabidopsis COP9 is a component of a novel signaling complex mediating light control of development.
    Cell. 1994 Jul 15;78(1):117-24 PMID: 8033203
  29. Mutations in the gene for the red/far-red light receptor phytochrome B alter cell elongation and physiological responses throughout Arabidopsis development.
    Plant Cell. 1993 Feb;5(2):147-57 PMID: 8453299
  30. Co-action between phytochrome B and HY4 in Arabidopsis thaliana.
    Planta. 1995;197(2):213-8 PMID: 8547813
  31. Mutations in the DET1 gene affect cell-type-specific expression of light-regulated genes and chloroplast development in Arabidopsis.
    Proc Natl Acad Sci U S A. 1990 Nov;87(22):8776-80 PMID: 2247447
  32. Isolation and Initial Characterization of Arabidopsis Mutants That Are Deficient in Phytochrome A.
    Plant Physiol. 1993 May;102(1):269-277 PMID: 12231818
  33. hy8, a new class of arabidopsis long hypocotyl mutants deficient in functional phytochrome A.
    Plant Cell. 1993 Jan;5(1):39-48 PMID: 8439743
  34. Arabidopsis COP8, COP10, and COP11 genes are involved in repression of photomorphogenic development in darkness.
    Plant Cell. 1994 May;6(5):629-43 PMID: 8038603
  35. Growth and development of the axr1 mutants of Arabidopsis.
    Plant Cell. 1990 Nov;2(11):1071-80 PMID: 1983791
  36. Arabidopsis thaliana mutant that develops as a light-grown plant in the absence of light.
    Cell. 1989 Sep 8;58(5):991-9 PMID: 2776216
  37. Phytochrome A and Phytochrome B Have Overlapping but Distinct Functions in Arabidopsis Development.
    Plant Physiol. 1994 Apr;104(4):1139-1149 PMID: 12232154
  38. The SAR1 gene of Arabidopsis acts downstream of the AXR1 gene in auxin response.
    Development. 1997 Apr;124(8):1583-91 PMID: 9108374
  39. Light control of plant development.
    Annu Rev Cell Dev Biol. 1997;13:203-29 PMID: 9442873
  40. Action spectra for phytochrome A- and B-specific photoinduction of seed germination in Arabidopsis thaliana.
    Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):8129-33 PMID: 8755615
  41. Two dominant photomorphogenic mutations of Arabidopsis thaliana identified as suppressor mutations of hy2.
    Plant J. 1996 Apr;9(4):441-56 PMID: 8624510
  42. DET1, a negative regulator of light-mediated development and gene expression in arabidopsis, encodes a novel nuclear-localized protein.
    Cell. 1994 Jul 15;78(1):109-16 PMID: 8033202
  43. Spotlight on Phytochrome Nomenclature.
    Plant Cell. 1994 Apr;6(4):468-471 PMID: 12244245
  44. Photoresponses of Light-Grown phyA Mutants of Arabidopsis (Phytochrome A Is Required for the Perception of Daylength Extensions).
    Plant Physiol. 1994 May;105(1):141-149 PMID: 12232194
  45. HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptor.
    Nature. 1993 Nov 11;366(6451):162-6 PMID: 8232555
  46. Structural and genetic analysis of epidermal cell differentiation in Arabidopsis primary roots.
    Development. 1997 May;124(9):1789-98 PMID: 9165126
  47. Phytochrome: a light-activated molecular switch that regulates plant gene expression.
    Annu Rev Genet. 1991;25:389-409 PMID: 1812812
  48. A brassinosteroid-insensitive mutant in Arabidopsis thaliana exhibits multiple defects in growth and development.
    Plant Physiol. 1996 Jul;111(3):671-8 PMID: 8754677
  49. Mutational analyses of light-controlled seedling development in Arabidopsis.
    Semin Cell Biol. 1994 Oct;5(5):327-34 PMID: 7881072
  50. Light inactivation of Arabidopsis photomorphogenic repressor COP1 involves a cell-specific regulation of its nucleocytoplasmic partitioning.
    Cell. 1994 Dec 16;79(6):1035-45 PMID: 8001131
  51. Mutations in the AXR3 gene of Arabidopsis result in altered auxin response including ectopic expression from the SAUR-AC1 promoter.
    Plant J. 1996 Sep;10(3):403-13 PMID: 8811856
  52. Arabidopsis HY8 locus encodes phytochrome A.
    Plant Cell. 1993 Sep;5(9):1081-8 PMID: 8400877
  53. The DIMINUTO gene of Arabidopsis is involved in regulating cell elongation.
    Genes Dev. 1995 Jan 1;9(1):97-107 PMID: 7828854
  54. Calcium/calmodulin-dependent and -independent phytochrome signal transduction pathways.
    Cell. 1993 Jun 4;73(5):937-52 PMID: 8388782
  55. A screen for genes that function downstream of Ras1 during Drosophila eye development.
    Genetics. 1996 May;143(1):315-29 PMID: 8722784
  56. A role for brassinosteroids in light-dependent development of Arabidopsis.
    Science. 1996 Apr 19;272(5260):398-401 PMID: 8602526
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/148.3.1295
Email
[email protected]
Published
1998-03-00
Pages
1295-310
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1460030
Subset
IM
Grants
NIGMS NIH HHS · R01-GM52413 · United States
NIGMS NIH HHS · R29-GM52456 · United States
External Links
PubMed source DOI: 10.1093/genetics/148.3.1295 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2