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PMID: 9539814 Published · ppublish English Journal Article

Architectural specificity in chromatin structure at the TATA box in vivo: nucleosome displacement upon beta-phaseolin gene activation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 8 ·1998-04-14 ·Pages 4772-7

Li G, Chandler SP, Wolffe AP, Hall TC

Abstract

Extensive studies of the beta-phaseolin (phas) gene in transgenic tobacco have shown that it is highly active during seed embryogenesis but is completely silent in leaf and other vegetative tissues. In vivo footprinting revealed that the lack of even basal transcriptional activity in vegetative tissues is associated with the presence of a nucleosome that is rotationally positioned with base pair precision over three phased TATA boxes present in the phas promoter. Positioning is sequence-dependent because an identical rotational setting is obtained upon nucleosome reconstitution in vitro. A comparison of DNase I and dimethyl sulfate footprints in vivo and in vitro strongly suggests that this repressive chromatin architecture is remodeled concomitant with gene activation in the developing seed. This leads to the disruption of histone-mediated DNA wrapping and the assembly of the TATA boxes into a transcriptionally competent nucleoprotein complex.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Li G
Institute of Developmental and Molecular Biology and Department of Biology, Texas A&M University, College Station, TX 77843-3155, USA.
Chandler S P
Wolffe A P
Hall T C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.8.4772
Published
1998-04-14
Pages
4772-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC22566
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1073/pnas.95.8.4772 PMC Full Text View journal details
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