Home LiteratureArticle Details
PMID: 9557689 Published · ppublish English Journal Article

The Epstein-Barr virus BARF1 gene encodes a novel, soluble colony-stimulating factor-1 receptor.

Journal of virology ·Vol. 72 ·No. 5 ·1998-05-00 ·Pages 4015-21

Strockbine LD, Cohen JI, Farrah T, Lyman SD, Wagener F, DuBose RF, Armitage RJ, Spriggs MK

Abstract

Epstein-Barr virus (EBV) is a ubiquitous herpesvirus associated with infectious mononucleosis and several tumors. The BARF1 gene is transcribed early after EBV infection from the BamHI A fragment of the EBV genome. Evidence shown here indicates that the BARF1 protein is secreted into the medium of transfected cells and from EBV-carrying B cells induced to allow lytic replication of the virus. Expression cloning identified colony-stimulating factor-1 (CSF-1) as a ligand for BARF1. Computer-assisted analyses indicated that subtle amino acid sequence homology exists between BARF1 and c-fins, the cellular proto-oncogene that is the receptor for CSF-1. Recombinant BARF1 protein was found to be biologically active, and it neutralized the proliferative effects of human CSF-1 in a dose-dependent fashion when assayed in vitro. Since CSF-1 is a pleiotropic cytokine best known for its differentiating effects on macrophages, these data suggest that BARF1 may function to modulate the host immune response to EBV infection.

MeSH Terms
Animals COS Cells Cell Line Chlorocebus aethiops Genes, Viral Herpesvirus 4, Human/genetics,metabolism Humans Ligands Macrophage Colony-Stimulating Factor/genetics,metabolism Proto-Oncogene Mas Receptor, Macrophage Colony-Stimulating Factor/genetics,metabolism Sequence Homology, Amino Acid Solubility Viral Proteins/genetics,metabolism
Chemicals
BARF1 protein, Human herpesvirus 4 Ligands MAS1 protein, human Proto-Oncogene Mas Viral Proteins Macrophage Colony-Stimulating Factor Receptor, Macrophage Colony-Stimulating Factor
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Strockbine L D
Immunex Corporation, Seattle, Washington 98101, USA.
Cohen J I
Farrah T
Lyman S D
Wagener F
DuBose R F
Armitage R J
Spriggs M K
References (56)
56 references, click to expand
  1. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  2. Epstein-Barr virus BCRF1 gene product (viral interleukin 10) inhibits superoxide anion production by human monocytes.
    Lymphokine Cytokine Res. 1992 Oct;11(5):209-14 PMID: 1334711
  3. Identifying nonpolar transbilayer helices in amino acid sequences of membrane proteins.
    Annu Rev Biophys Biophys Chem. 1986;15:321-53 PMID: 3521657
  4. Macrophage growth factor CSF-1 stimulates human monocyte production of interferon, tumor necrosis factor, and colony stimulating activity.
    J Immunol. 1986 Oct 1;137(7):2281-5 PMID: 2428865
  5. Transforming potential of the c-fms proto-oncogene (CSF-1 receptor).
    Nature. 1987 Feb 5-11;325(6104):549-52 PMID: 3027579
  6. Human CSF-1: molecular cloning and expression of 4-kb cDNA encoding the human urinary protein.
    Science. 1987 Mar 20;235(4795):1504-8 PMID: 3493529
  7. Altered expression of two Epstein-Barr virus early genes localized in BamHI-A in nonproducer Raji cells.
    J Virol. 1988 Jun;62(6):1862-9 PMID: 2835494
  8. Macrophage colony-stimulating factor is produced by human T lymphoblastoid cell line, CEM-ON: identification by amino-terminal amino acid sequence analysis.
    Biochem Biophys Res Commun. 1988 May 16;152(3):1401-9 PMID: 3259875
  9. cDNA expression cloning of the IL-1 receptor, a member of the immunoglobulin superfamily.
    Science. 1988 Jul 29;241(4865):585-9 PMID: 2969618
  10. Human macrophage-colony stimulating factor: alternative RNA and protein processing from a single gene.
    Mol Immunol. 1988 Aug;25(8):761-70 PMID: 2460758
  11. A point mutation in the extracellular domain of the human CSF-1 receptor (c-fms proto-oncogene product) activates its transforming potential.
    Cell. 1988 Dec 23;55(6):979-88 PMID: 2974321
  12. The murine interleukin-4 receptor: molecular cloning and characterization of secreted and membrane bound forms.
    Cell. 1989 Oct 20;59(2):335-48 PMID: 2805066
  13. Mouse NIH 3T3 cells expressing human colony-stimulating factor 1 (CSF-1) receptors overgrow in serum-free medium containing human CSF-1 as their only growth factor.
    Proc Natl Acad Sci U S A. 1989 Oct;86(20):7924-7 PMID: 2554296
  14. A transforming function of the BARF1 gene encoded by Epstein-Barr virus.
    EMBO J. 1989 Oct;8(10):2897-903 PMID: 2555151
  15. Colony-stimulating factor-1 receptor.
    Blood. 1990 Jan 1;75(1):1-12 PMID: 2153029
  16. A point mutation at tyrosine-809 in the human colony-stimulating factor 1 receptor impairs mitogenesis without abrogating tyrosine kinase activity, association with phosphatidylinositol 3-kinase, or induction of c-fos and junB genes.
    Proc Natl Acad Sci U S A. 1990 Sep;87(17):6738-42 PMID: 2168557
  17. Both granulocyte-macrophage colony-stimulating factor and monocytic colony-stimulating factor are produced by the human T-cell line, HUT 102.
    Exp Hematol. 1990 Nov;18(10):1090-3 PMID: 2145180
  18. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  19. Macrophage colony-stimulating factor (CSF-1) gene expression in human T-lymphocyte clones.
    Blood. 1991 Feb 15;77(4):780-6 PMID: 1993220
  20. Granulocyte-macrophage and macrophage colony-stimulating factors differentially regulate alpha v integrin expression on cultured human macrophages.
    Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2517-21 PMID: 7681600
  21. Interleukin-10.
    Annu Rev Immunol. 1993;11:165-90 PMID: 8386517
  22. Macrophage colony-stimulating factor (M-CSF) enhances complement component C3 production by human monocytes/macrophages.
    Int J Hematol. 1993 Jan;57(1):53-9 PMID: 8477063
  23. Identification of the ligand-binding regions in the macrophage colony-stimulating factor receptor extracellular domain.
    Mol Cell Biol. 1993 Sep;13(9):5348-59 PMID: 8355686
  24. Epstein-Barr virus-coded BHRF1 protein, a viral homologue of Bcl-2, protects human B cells from programmed cell death.
    Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8479-83 PMID: 8397406
  25. Production of colony-stimulating factor 1 by T cells: possible involvement in their interaction with antigen-presenting cells.
    Cytokine. 1993 Jul;5(4):309-18 PMID: 8260596
  26. Expression and tumorigenicity of the Epstein-Barr virus BARF1 gene in human Louckes B-lymphocyte cell line.
    Cancer Res. 1994 Apr 1;54(7):1843-8 PMID: 8137299
  27. Epstein-Barr virus BHRF1 protein protects against cell death induced by DNA-damaging agents and heterologous viral infection.
    Virology. 1994 Jun;201(2):404-7 PMID: 8184552
  28. Molecular characterization of murine and human OX40/OX40 ligand systems: identification of a human OX40 ligand as the HTLV-1-regulated protein gp34.
    EMBO J. 1994 Sep 1;13(17):3992-4001 PMID: 8076595
  29. Molecular and biological characterization of human 4-1BB and its ligand.
    Eur J Immunol. 1994 Sep;24(9):2219-27 PMID: 8088337
  30. The immunoglobulin fold. Structural classification, sequence patterns and common core.
    J Mol Biol. 1994 Sep 30;242(4):309-20 PMID: 7932691
  31. Structural analyses of the Epstein-Barr virus BamHI A transcripts.
    J Virol. 1995 Feb;69(2):1132-41 PMID: 7815492
  32. Ligands for the receptor tyrosine kinases hek and elk: isolation of cDNAs encoding a family of proteins.
    Oncogene. 1995 Jan 19;10(2):299-306 PMID: 7838529
  33. Identification of OX40 ligand and preliminary characterization of its activities on OX40 receptor.
    Circ Shock. 1994 Sep;44(1):30-4 PMID: 7704935
  34. Vaccinia virus encodes a soluble type I interferon receptor of novel structure and broad species specificity.
    Cell. 1995 May 19;81(4):551-60 PMID: 7758109
  35. Vaccinia virus B18R gene encodes a type I interferon-binding protein that blocks interferon alpha transmembrane signaling.
    J Biol Chem. 1995 Jul 7;270(27):15974-8 PMID: 7608155
  36. The lytic cycle of Epstein-Barr virus in the nonproducer Raji line can be rescued by the expression of a 135-kilodalton protein encoded by the BALF2 open reading frame.
    J Virol. 1995 Nov;69(11):7309-14 PMID: 7474160
  37. Interleukin-10 and its receptor.
    Ther Immunol. 1994 Jun;1(3):173-85 PMID: 7584493
  38. Transcriptional expression of Epstein-Barr virus genes and proto-oncogenes in north African nasopharyngeal carcinoma.
    J Med Virol. 1996 May;49(1):7-14 PMID: 8732865
  39. The extracellular domain of the Epstein-Barr virus BZLF2 protein binds the HLA-DR beta chain and inhibits antigen presentation.
    J Virol. 1996 Aug;70(8):5557-63 PMID: 8764069
  40. Conserved linkage between the puffer fish (Fugu rubripes) and human genes for platelet-derived growth factor receptor and macrophage colony-stimulating factor receptor.
    Genome Res. 1996 Dec;6(12):1185-91 PMID: 8973913
  41. Biology and action of colony--stimulating factor-1.
    Mol Reprod Dev. 1997 Jan;46(1):4-10 PMID: 8981357
  42. Antibody and antibody-dependent cellular cytotoxicity responses against the BamHI A rightward open-reading frame-1 protein of Epstein-Barr virus (EBV) in EBV-associated disorders.
    J Infect Dis. 1997 Jan;175(1):38-46 PMID: 8985194
  43. The purified myxoma virus gamma interferon receptor homolog M-T7 interacts with the heparin-binding domains of chemokines.
    J Virol. 1997 Jun;71(6):4356-63 PMID: 9151824
  44. Establishment of a monkey kidney epithelial cell line with the BARF1 open reading frame from Epstein-Barr virus.
    Oncogene. 1997 Jun 26;14(25):3073-81 PMID: 9223671
  45. CSF-I signal transduction: what is of functional significance?
    Immunol Today. 1997 Jul;18(7):313-7 PMID: 9238832
  46. Poxvirus genomes encode a secreted, soluble protein that preferentially inhibits beta chemokine activity yet lacks sequence homology to known chemokine receptors.
    Virology. 1997 Sep 29;236(2):316-27 PMID: 9325239
  47. One step ahead of the game: viral immunomodulatory molecules.
    Annu Rev Immunol. 1996;14:101-30 PMID: 8717509
  48. Regulation of interleukin 4 receptors on human T cells.
    Int Immunol. 1990;2(11):1039-45 PMID: 2083226
  49. A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types.
    EMBO J. 1991 Oct;10(10):2821-32 PMID: 1833184
  50. Expression of the Epstein-Barr virus BamHI A fragment in nasopharyngeal carcinoma: evidence for a viral protein expressed in vivo.
    J Virol. 1991 Nov;65(11):6252-9 PMID: 1656092
  51. Myc rescue of a mutant CSF-1 receptor impaired in mitogenic signalling.
    Nature. 1991 Sep 26;353(6342):361-3 PMID: 1833648
  52. Molecular and biological characterization of a murine ligand for CD40.
    Nature. 1992 May 7;357(6373):80-2 PMID: 1374165
  53. Soluble forms of CD40 inhibit biologic responses of human B cells.
    J Immunol. 1992 Jul 15;149(2):655-60 PMID: 1378075
  54. Transcription of BamHI-A region of the EBV genome in NPC tissues and B cells.
    Virology. 1992 Nov;191(1):193-201 PMID: 1329317
  55. Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion.
    J Exp Med. 1992 Dec 1;176(6):1543-50 PMID: 1281209
  56. Epstein-Barr virus transcription in nasopharyngeal carcinoma.
    J Virol. 1983 Dec;48(3):580-90 PMID: 6313960
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.72.5.4015-4021.1998
Published
1998-05-00
Pages
4015-21
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC109629
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.72.5.4015-4021.1998 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2