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PMID: 9566905 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Axil, a member of the Axin family, interacts with both glycogen synthase kinase 3beta and beta-catenin and inhibits axis formation of Xenopus embryos.

Molecular and cellular biology ·Vol. 18 ·No. 5 ·1998-05-00 ·Pages 2867-75

Yamamoto H, Kishida S, Uochi T, Ikeda S, Koyama S, Asashima M, Kikuchi A

Abstract

Using a yeast two-hybrid method, we identified a novel protein which interacts with glycogen synthase kinase 3beta (GSK-3beta). This protein had 44% amino acid identity with Axin, a negative regulator of the Wnt signaling pathway. We designated this protein Axil for Axin like. Like Axin, Axil ventralized Xenopus embryos and inhibited Xwnt8-induced Xenopus axis duplication. Axil was phosphorylated by GSK-3beta. Axil bound not only to GSK-3beta but also to beta-catenin, and the GSK-3beta-binding site of Axil was distinct from the beta-catenin-binding site. Furthermore, Axil enhanced GSK-3beta-dependent phosphorylation of beta-catenin. These results indicate that Axil negatively regulates the Wnt signaling pathway by mediating GSK-3beta-dependent phosphorylation of beta-catenin, thereby inhibiting axis formation.

MeSH Terms
Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Axin Protein Body Patterning Calcium-Calmodulin-Dependent Protein Kinases/metabolism Carrier Proteins/genetics,metabolism Cytoskeletal Proteins/metabolism Glycogen Synthase Kinase 3 Glycogen Synthase Kinases Molecular Sequence Data Phosphorylation Protein Binding Proteins/genetics,metabolism Repressor Proteins Sequence Homology, Amino Acid Signal Transduction Trans-Activators Wnt Proteins Xenopus/embryology Xenopus Proteins Zebrafish Proteins beta Catenin
Chemicals
AXIN2 protein, Xenopus Adaptor Proteins, Signal Transducing Axin Protein CTNNB1 protein, Xenopus Carrier Proteins Cytoskeletal Proteins Proteins Repressor Proteins Trans-Activators Wnt Proteins Xenopus Proteins Zebrafish Proteins axin1 protein, Xenopus beta Catenin wnt8a protein, zebrafish Glycogen Synthase Kinases Calcium-Calmodulin-Dependent Protein Kinases Glycogen Synthase Kinase 3
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Yamamoto H
Department of Biochemistry, Hiroshima University School of Medicine, Japan.
Kishida S
Uochi T
Ikeda S
Koyama S
Asashima M
Kikuchi A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.5.2867
Published
1998-05-00
Pages
2867-75
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC110665
Subset
IM
Databases
GENBANK
AF017757
External Links
PubMed source DOI: 10.1128/MCB.18.5.2867 PMC Full Text View journal details
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