Abstract
Identification of enterococci by using a semiautomated system (PASCO; Difco Laboratories, Detroit, Mich.) in the AHEPA University Hospital, Thessaloniki, Greece, revealed a high proportion of Enterococcus durans, particularly among isolates highly resistant to gentamicin and streptomycin. When 14 isolates were further tested by a conventional biochemical scheme and by PCR, all were reidentified as Enterococcus faecalis. Antibiotic resistance and pulsed-field gel electrophoresis patterns showed that unrelated strains were misidentified.
MeSH Terms
Anti-Bacterial Agents/pharmacology
DNA, Bacterial/drug effects
Drug Resistance, Microbial
Enterococcus/classification,drug effects,genetics
Gentamicins/pharmacology
Software
Streptomycin/pharmacology
Chemicals
Anti-Bacterial Agents
DNA, Bacterial
Gentamicins
Streptomycin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Tsakris A
Department of Microbiology, Medical School, Aristotelian University of Thessaloniki, Greece.
[email protected]
Woodford N
Pournaras S
Kaufmann M
Douboyas J
References (16)
16 references, click to expand
-
Identification of Enterococcus species isolated from human infections by a conventional test scheme.
J Clin Microbiol. 1989 Apr;27(4):731-4
PMID: 2656745
-
Pseudo-outbreak of Enterococcus durans infections and colonization associated with introduction of an automated identification system software update.
J Clin Microbiol. 1996 Nov;34(11):2685-7
PMID: 8897165
-
Species identities of enterococci isolated from clinical specimens.
J Clin Microbiol. 1990 Mar;28(3):435-7
PMID: 2108992
-
Evaluation of MicroScan for identification of Enterococcus species.
J Clin Microbiol. 1990 Jun;28(6):1477-8
PMID: 2116449
-
Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites.
J Clin Microbiol. 1990 Sep;28(9):2059-63
PMID: 1977766
-
Intrageneric relationships of Enterococci as determined by reverse transcriptase sequencing of small-subunit rRNA.
Res Microbiol. 1991 Jan;142(1):67-74
PMID: 1712504
-
Vancomycin susceptibility and identification of motile enterococci.
J Clin Microbiol. 1991 Oct;29(10):2335-7
PMID: 1939593
-
High-level resistance to gentamicin in Enterococcus faecium.
J Antimicrob Chemother. 1992 Apr;29(4):395-403
PMID: 1607328
-
Antimicrobial susceptibility patterns of common and unusual species of enterococci causing infections in the United States. Enterococcal Study Group.
J Clin Microbiol. 1992 Sep;30(9):2373-8
PMID: 1401001
-
Rapid increase in the prevalence of high-level aminoglycoside resistance among enterococci isolated from blood cultures during 1989-1991.
J Antimicrob Chemother. 1992 Sep;30(3):289-93
PMID: 1452493
-
Evaluation and characterization of multiresistant Enterococcus faecium from 12 U.S. medical centers.
J Clin Microbiol. 1994 Nov;32(11):2840-2
PMID: 7852582
-
Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR.
J Clin Microbiol. 1995 Jan;33(1):24-7
PMID: 7699051
-
Enterococci: susceptibility patterns and therapeutic options.
Eur J Clin Microbiol Infect Dis. 1995;14 Suppl 1:S33-7
PMID: 7729470
-
Prevalence of antibiotic resistance among enterococci isolated in Greece.
J Antimicrob Chemother. 1995 Jan;35(1):237-8
PMID: 7768780
-
Survey of enterococcal susceptibility patterns in Belgium.
J Clin Microbiol. 1996 Oct;34(10):2572-6
PMID: 8880522
-
The life and times of the Enterococcus.
Clin Microbiol Rev. 1990 Jan;3(1):46-65
PMID: 2404568