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PMID: 9574718 Published · ppublish English Journal Article

Apparent increased prevalence of high-level aminoglycoside-resistant Enterococcus durans resulting from false identification by a semiautomated software system.

Journal of clinical microbiology ·Vol. 36 ·No. 5 ·1998-05-00 ·Pages 1419-21

Tsakris A, Woodford N, Pournaras S, Kaufmann M, Douboyas J

Abstract

Identification of enterococci by using a semiautomated system (PASCO; Difco Laboratories, Detroit, Mich.) in the AHEPA University Hospital, Thessaloniki, Greece, revealed a high proportion of Enterococcus durans, particularly among isolates highly resistant to gentamicin and streptomycin. When 14 isolates were further tested by a conventional biochemical scheme and by PCR, all were reidentified as Enterococcus faecalis. Antibiotic resistance and pulsed-field gel electrophoresis patterns showed that unrelated strains were misidentified.

MeSH Terms
Anti-Bacterial Agents/pharmacology DNA, Bacterial/drug effects Drug Resistance, Microbial Enterococcus/classification,drug effects,genetics Gentamicins/pharmacology Software Streptomycin/pharmacology
Chemicals
Anti-Bacterial Agents DNA, Bacterial Gentamicins Streptomycin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Tsakris A
Department of Microbiology, Medical School, Aristotelian University of Thessaloniki, Greece. [email protected]
Woodford N
Pournaras S
Kaufmann M
Douboyas J
References (16)
16 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
Published
1998-05-00
Pages
1419-21
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC104841
Subset
IM
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