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PMID: 9596741 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Strain-dependent activation of monocytes and inflammatory macrophages by lipopolysaccharide of Porphyromonas gingivalis.

Infection and immunity ·Vol. 66 ·No. 6 ·1998-06-00 ·Pages 2736-42

Shapira L, Champagne C, Van Dyke TE, Amar S

Abstract

Porphyromonas gingivalis is one of the pathogens associated with periodontal diseases, and its lipopolysaccharide (LPS) has been suggested as a possible virulence factor, acting by stimulation of host cells to secrete proinflammatory mediators. However, recent studies have shown that P. gingivalis LPS inhibited some components of the inflammatory response. The present study was designed to test the hypothesis that there are strain-dependent variations in the ability of P. gingivalis LPS to elicit the host inflammatory response. By using LPS preparations from two strains of P. gingivalis, W50 and A7346, the responses of mouse macrophages and human monocytes were evaluated by measuring the secretion of nitric oxide (NO) and tumor necrosis factor alpha (TNF-alpha). Both direct and indirect (priming) effects were investigated. LPS from Salmonella typhosa was used as a reference LPS. P. gingivalis A7436 LPS induced lower secreted levels of NO from the tested cells than S. typhosa LPS but induced similar levels of TNF-alpha. In contrast, LPS from P. gingivalis W50 did not induce NO or TNF-alpha secretion. Preincubation of macrophages with LPS from S. typhosa or P. gingivalis A7436 prior to stimulation with S. typhosa LPS upregulated NO secretion and downregulated TNF-alpha secretion, while preincubation with P. gingivalis W50 LPS enhanced both TNF-alpha and NO secretory responses. These results demonstrate that LPSs derived from different strains of P. gingivalis vary in their biological activities in vitro. The findings may have an impact on our understanding of the range of P. gingivalis virulence in vivo.

MeSH Terms
Animals Cell Division Electrophoresis, Polyacrylamide Gel Female Humans Lipopolysaccharides/immunology Macrophage Activation Macrophages/cytology,immunology Mice Mice, Inbred BALB C Monocytes/cytology,immunology Nitric Oxide/metabolism Porphyromonas gingivalis/immunology Species Specificity Tumor Necrosis Factor-alpha/metabolism
Chemicals
Lipopolysaccharides Tumor Necrosis Factor-alpha Nitric Oxide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shapira L
Department of Periodontology, Hebrew University-Hadassah School of Dental Medicine, Jerusalem, Israel. [email protected]
Champagne C
Van Dyke T E
Amar S
References (44)
44 references, click to expand
  1. Lipopolysaccharide (endotoxin) from individual periodontally involved teeth.
    J Clin Periodontol. 1987 Sep;14(8):453-6 PMID: 3477573
  2. Identity of limulus amoebocyte lysate-active root surface materials from periodontally involved teeth.
    J Clin Periodontol. 1986 Sep;13(8):743-7 PMID: 3464618
  3. Biology of asaccharolytic black-pigmented Bacteroides species.
    Microbiol Rev. 1988 Mar;52(1):134-52 PMID: 3280965
  4. Detection of circulating tumor necrosis factor after endotoxin administration.
    N Engl J Med. 1988 Jun 9;318(23):1481-6 PMID: 2835680
  5. Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production.
    J Immunol. 1988 Oct 1;141(7):2407-12 PMID: 3139757
  6. Lipopolysaccharide-stimulated PGE2 release from human monocytes. Comparison of lipopolysaccharides prepared from suspected periodontal pathogens.
    J Periodontol. 1988 Oct;59(10):684-7 PMID: 3054046
  7. The cardiovascular response of normal humans to the administration of endotoxin.
    N Engl J Med. 1989 Aug 3;321(5):280-7 PMID: 2664516
  8. Increased interleukin-1 beta (IL-1 beta) concentration in gingival tissue from periodontitis patients.
    J Periodontal Res. 1989 Nov;24(6):362-7 PMID: 2531790
  9. Role of tumor necrosis factor-alpha in lipopolysaccharide-induced pathologic alterations.
    Am J Pathol. 1990 Jan;136(1):49-60 PMID: 2297050
  10. Chemical characterization and biologic properties of lipopolysaccharide from Bacteroides gingivalis strains W50, W83, and ATCC 33277.
    Oral Microbiol Immunol. 1989 Dec;4(4):183-92 PMID: 2640312
  11. A novel mouse model to study the virulence of and host response to Porphyromonas (Bacteroides) gingivalis.
    Infect Immun. 1991 Apr;59(4):1255-63 PMID: 2004807
  12. Detection of interleukin 8 and tumor necrosis factor in normal humans after intravenous endotoxin: the effect of antiinflammatory agents.
    J Exp Med. 1991 Apr 1;173(4):1021-4 PMID: 2007851
  13. Serum levels of tumor necrosis factor determine the fatal or non-fatal course of endotoxic shock.
    Immunol Lett. 1991 Feb;27(2):157-62 PMID: 2026458
  14. The distribution of bacterial lipopolysaccharide (endotoxin) in relation to periodontally involved root surfaces.
    J Clin Periodontol. 1986 Sep;13(8):748-51 PMID: 3464619
  15. Inflammatory mediators and the destruction of bone.
    J Periodontal Res. 1991 May;26(3 Pt 2):213-7 PMID: 1831844
  16. Levels of interleukin 1 beta in tissue from sites of active periodontal disease.
    J Clin Periodontol. 1991 Aug;18(7):548-54 PMID: 1894750
  17. Factors in virulence expression and their role in periodontal disease pathogenesis.
    Crit Rev Oral Biol Med. 1991;2(2):177-281 PMID: 1912148
  18. Tissue levels of bone resorptive cytokines in periodontal disease.
    J Periodontol. 1991 Aug;62(8):504-9 PMID: 1920018
  19. The bacterial etiology of destructive periodontal disease: current concepts.
    J Periodontol. 1992 Apr;63(4 Suppl):322-31 PMID: 1573546
  20. Time course of various inflammatory mediators during recurrent endotoxemia.
    Biochem Pharmacol. 1992 May 28;43(10):2103-9 PMID: 1599496
  21. Endotoxin levels in periodontally healthy and diseased sites: correlation with levels of gram-negative bacteria.
    J Periodontol. 1992 Nov;63(11):897-901 PMID: 1453305
  22. Lipopolysaccharide-induced selective priming effects on tumor necrosis factor alpha and nitric oxide production in mouse peritoneal macrophages.
    J Exp Med. 1993 Feb 1;177(2):511-6 PMID: 8426119
  23. Response of man to endotoxin.
    Immunobiology. 1993 Apr;187(3-5):403-16 PMID: 8330905
  24. The role of the host response in periodontal disease progression: implications for future treatment strategies.
    J Periodontol. 1993 Aug;64(8 Suppl):792-806 PMID: 8410619
  25. Immunobiological properties of chemically defined lipid A from lipopolysaccharide of Porphyromonas (Bacteroides) gingivalis.
    Eur J Biochem. 1994 Feb 1;219(3):737-42 PMID: 8112323
  26. The secretion of PGE2, IL-1 beta, IL-6, and TNF alpha by adherent mononuclear cells from early onset periodontitis patients.
    J Periodontol. 1994 Feb;65(2):139-46 PMID: 8158510
  27. Porphyromonas gingivalis lipopolysaccharide stimulation of human monocytes: dependence on serum and CD14 receptor.
    Oral Microbiol Immunol. 1994 Apr;9(2):112-7 PMID: 7516534
  28. Immunobiological activities of chemically defined lipid A from lipopolysaccharides of Porphyromonas gingivalis.
    Microbiology. 1994 May;140 ( Pt 5):1209-16 PMID: 8025687
  29. Involvement of protein kinase C and protein tyrosine kinase in lipopolysaccharide-induced TNF-alpha and IL-1 beta production by human monocytes.
    J Immunol. 1994 Aug 15;153(4):1818-24 PMID: 7519214
  30. Animal chamber models for study of host-parasite interactions.
    Methods Enzymol. 1994;235:120-40 PMID: 8057891
  31. Effects of a Porphyromonas gingivalis infection on inflammatory mediator response and pregnancy outcome in hamsters.
    Infect Immun. 1994 Oct;62(10):4356-61 PMID: 7927695
  32. Ability of bacteria associated with chronic inflammatory disease to stimulate E-selectin expression and promote neutrophil adhesion.
    Infect Immun. 1995 Apr;63(4):1311-7 PMID: 7534275
  33. Porphyromonas gingivalis lipopolysaccharide is poorly recognized by molecular components of innate host defense in a mouse model of early inflammation.
    Infect Immun. 1995 Dec;63(12):4686-94 PMID: 7591124
  34. Protection against endotoxic shock and lipopolysaccharide-induced local inflammation by tetracycline: correlation with inhibition of cytokine secretion.
    Infect Immun. 1996 Mar;64(3):825-8 PMID: 8641787
  35. Immunomodulatory and superantigen activities of bacteria associated with adult periodontitis.
    J Periodontol. 1996 Sep;67(9):909-17 PMID: 8884649
  36. Lipopolysaccharide isolated from Porphyromonas gingivalis grown in hemin-limited chemostat conditions has a reduced capacity for human neutrophil priming.
    Oral Microbiol Immunol. 1996 Oct;11(5):319-25 PMID: 9028257
  37. Tumor necrosis factor (TNF)-induced cutaneous necrosis is mediated by TNF receptor 1.
    J Inflamm. 1995-1996;47(4):180-9 PMID: 9144075
  38. The presence and biologic activity of cementum-bound endotoxin.
    J Periodontol. 1974 Sep;45(9):672-5 PMID: 4529435
  39. Fractions of lipopolysaccharide from Escherichia coli O111:B4 prepared by two extraction procedures.
    J Biol Chem. 1975 Apr 25;250(8):2911-19 PMID: 804483
  40. Stimulation of macromolecular synthesis by endotoxin-treated 3T6 fibroblasts.
    Experientia. 1980 May 15;36(5):546-7 PMID: 6247193
  41. Preliminary characterization of material eluted from the roots of periodontally diseased teeth.
    J Periodontal Res. 1980 Jan;15(1):10-9 PMID: 6445966
  42. Modulation of bone metabolism by two chemically distinct lipopolysaccharide fractions from Bacteroides gingivalis.
    Infect Immun. 1986 Jan;51(1):302-6 PMID: 3940999
  43. The use of crevicular fluid prostaglandin E2 levels as a predictor of periodontal attachment loss.
    J Periodontal Res. 1986 Mar;21(2):101-12 PMID: 2937899
  44. Cytokine appearance in human endotoxemia and primate bacteremia.
    Surg Gynecol Obstet. 1988 Feb;166(2):147-53 PMID: 3122336
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.66.6.2736-2742.1998
Email
[email protected]
Published
1998-06-00
Pages
2736-42
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC108263
Subset
IM
Grants
NIDCR NIH HHS · DE-10709 · United States
NIDCR NIH HHS · DE06436 · United States
External Links
PubMed source DOI: 10.1128/IAI.66.6.2736-2742.1998 PMC Full Text View journal details
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