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PMID: 9603846 Published · ppublish English Journal Article

Estimation of nitrifying bacterial activities by measuring oxygen uptake in the presence of the metabolic inhibitors allylthiourea and azide

Applied and environmental microbiology ·Vol. 64 ·No. 6 ·1998-06-00 ·Pages 2266-8

Ginestet P, Audic JM, Urbain V, Block JC

Abstract

The effects of two metabolic inhibitors on an enriched nitrifying biomass during incubation for short periods of time were investigated by determining respirometric measurements. Allylthiourea (86 &mgr;M) and azide (24 &mgr;M) were shown to be strong, selective inhibitors of ammonia and nitrite oxidation, respectively. Consequently, a differential respirometry method for estimating nitrifying and heterotrophic bacterial activities within a mixed biomass is proposed.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ginestet
Centre International de Recherche sur l'Eau et l'Environment-Lyonnaise des Eaux, Le Pecq, France.
Audic
Urbain
Block
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10 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
1098-5336
DOI
10.1128/AEM.64.6.2266-2268.1998
Published
1998-06-00
Pages
2266-8
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC106310
External Links
PubMed source DOI: 10.1128/AEM.64.6.2266-2268.1998 PMC Full Text View journal details
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