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PMID: 96089 Published · ppublish English Journal Article

Early-blocked asporogenous mutants of Bacillus subtilis are lysogenized at reduced frequency by temperate bacteriophages.

Journal of bacteriology ·Vol. 134 ·No. 2 ·1978-05-00 ·Pages 440-5

Ikeuchi T, Kurahashi K

Abstract

The establishment of lysogeny in early-blocked asporogenous (Spo-) mutants of Bacillus subtilis 168, which were also defective in the production of antibiotics (Abs-), by temperate phage phi105 or SPO2 was studied. It was found that the frequency of lysogenization of Spo-Abs-mutants was 10 to 20% that of the wild-type bacteria. There was no difference in the efficiency of plating and the burst size of phi105 between wild-type and mutant strains. Phi105 lysogens of mutant strains were as stable as those of the wild type. Several rifampin-resistant mutants defective in the production of antibiotics were isolated. They were also defective in spore formation and lysogenized by phi105 at reduced frequency.

MeSH Terms
Anti-Bacterial Agents/biosynthesis Bacillus subtilis/genetics,physiology Bacteriophages/growth & development Chromosome Mapping Genes Lysogeny Mutation Rifampin/pharmacology Spores, Bacterial
Chemicals
Anti-Bacterial Agents Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ikeuchi T
Kurahashi K
References (35)
35 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.134.2.440-445.1978
Published
1978-05-00
Pages
440-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC222271
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.134.2.440-445.1978 PMC Full Text View journal details
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