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PMID: 9614233 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Changes in mitochondrial function resulting from synaptic activity in the rat hippocampal slice.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 18 ·No. 12 ·1998-06-15 ·Pages 4570-87

Bindokas VP, Lee CC, Colmers WF, Miller RJ

Abstract

Digital imaging microfluorimetry was used to visualize changes in mitochondrial potential and intracellular Ca2+ concentration, [Ca2+]i, in thick slices of rat hippocampus. Electrical activity, especially stimulus train-induced bursting (STIB) activity, produced slow, prolonged changes in mitochondrial potential within hippocampal slices as revealed by fluorescence measurements with rhodamine dyes. Changes in mitochondrial potential showed both temporal and spatial correlations with the intensity of the electrical activity. Patterned changes in mitochondrial potential were observed to last from tens of seconds to minutes as the consequence of epileptiform discharges. STIB-associated elevations in [Ca2+]i were also prolonged and exhibited a spatial pattern similar to that of the mitochondrial depolarization. The mitochondrial depolarization was sensitive to TTX and glutamate receptor blockers ([Mg2+]o and CNQX or DNQX plus D-AP-5) and to the inhibition of glutamate release by activation of presynaptic NPY receptors. The monitoring of mitochondrial potential in slice preparations provides a new tool for mapping synaptic activity in the brain and for determining the roles of mitochondria in regulation of brain synaptic activity.

MeSH Terms
Animals Calcium/metabolism Electrophysiology Hippocampus/physiology In Vitro Techniques Male Mitochondria/drug effects,physiology Osmolar Concentration Peptide YY/pharmacology Rats Rats, Sprague-Dawley Synapses/physiology
Chemicals
Peptide YY Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bindokas V P
Department of Pharmacological and Physiological Sciences, University of Chicago, Chicago, Illinois 60637, USA.
Lee C C
Colmers W F
Miller R J
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1998-06-15
Pages
4570-87
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6792701
Subset
IM
Grants
NICHD NIH HHS · T32 HD007009 · United States
NIGMS NIH HHS · T32 GM007151 · United States
NIDDK NIH HHS · P30 DK042086 · United States
NIDA NIH HHS · DA-02121 · United States
NIDA NIH HHS · DA-02575 · United States
NIMH NIH HHS · MH-40165 · United States
NIMH NIH HHS · R37 MH040165 · United States
External Links
PubMed source PMC Full Text View journal details
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