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PMID: 9615457 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nucleotide variation in the triosephosphate isomerase (Tpi) locus of Drosophila melanogaster and Drosophila simulans.

Molecular biology and evolution ·Vol. 15 ·No. 6 ·1998-06-00 ·Pages 756-69

Hasson E, Wang IN, Zeng LW, Kreitman M, Eanes WF

Abstract

DNA sequence variation in a 1.1-kb region including the coding portion of the Tpi locus was examined in 25 homozygous third-chromosome lines of Drosophila melanogaster, nine lines of Drosophila simulans, and one line of Drosophila yakuba. Our data show that the widespread allozyme polymorphism observed in cosmopolitan D. melanogaster is due to a glutamic acid substitution occurring in a phylogenetically conserved lysine that has been identified as part of the "hinged-lid" active site of the enzyme. This observation suggests that the replacement polymorphism may have important functional consequences. One replacement polymorphism was also observed in D. simulans, although its functional relevance is more difficult to assess, since it affects a site that is not strongly conserved. This amino acid change in D. simulans is associated with a single lineage possessing seven unique silent substitutions, which may be indicative of balancing selection or population subdivision. The absence of fixed amino acid differences between D. melanogaster and D. simulans and only a single difference with D. yakuba suggests that triose phosphate isomerase is under strong functional constraint. Silent variation is slightly higher for D. melanogaster than for D. simulans. Finally, we outline the general lack of evidence for old balanced polymorphisms at allozyme loci in D. melanogaster.

MeSH Terms
Alleles Animals Base Sequence Codon/genetics DNA/genetics Drosophila/genetics Drosophila melanogaster/genetics Gene Frequency Genes, Insect Genetic Variation Insect Proteins/genetics Isoenzymes/genetics Molecular Sequence Data Mutation Phylogeny Polymerase Chain Reaction Polymorphism, Genetic Recombination, Genetic Sequence Alignment Sequence Homology, Nucleic Acid Triose-Phosphate Isomerase/genetics
Chemicals
Codon Insect Proteins Isoenzymes DNA Triose-Phosphate Isomerase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hasson E
Department of Ecology and Evolution, State University of New York, Stony Brook 11794, USA.
Wang I N
Zeng L W
Kreitman M
Eanes W F
Article Info
Journal
Molecular biology and evolution
Abbr.
Mol Biol Evol
ISSN
0737-4038
DOI
10.1093/oxfordjournals.molbev.a025979
Published
1998-06-00
Pages
756-69
Language
English
Region
United States
NLM ID
8501455
Subset
IM
Grants
NIGMS NIH HHS · GM39355 · United States
NIGMS NIH HHS · GM45247 · United States
Databases
GENBANK
U60836, U60837, U60838, U60839, U60840, U60841, U60842, U60843, U60844, U60845, U60846, U60847, U60848, U60849, U60850, U60851, U60852, U60853, U60854, U60855, U60856, U60857, U60858, U60859, U60860, U60861, U60862, U60863, U60864, U60865
External Links
PubMed source DOI: 10.1093/oxfordjournals.molbev.a025979 View journal details
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