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PMID: 9620 Published · ppublish English Journal Article

Polynucleotides. XL. Synthesis and properties of poly 2'-azido-2'-deoxyadenylic acid.

Nucleic acids research ·Vol. 3 ·No. 8 ·1976-08-00 ·Pages 2089-99

Ikehara M, Fukui T, Kakiuchi N

Abstract

Poly 2'-azido-2'-deoxyadenylic acid (Poly Az) was synthesized from 2'-azido-2'-deoxyadenosine diphosphate by polynucleotide phosphorylase. Poly (Az) has U.V. absorption properties similar to poly (A) and hypochromicity of 40% at 0.1 M Na+ and neutrality. CD curve also resembled to that of poly (A), but has smaller ellipticity. Titration of poly (Az) with HCl gave a transition at pH 5.5, but exact structure of the acid-form complex was not elucidated. Upon mixing with poly (U), poly (Az) forms a 1:1 and 1:2 complexes having Tm's somewhat higher than that of poly (A)- poly (U) complex in the same condition.

MeSH Terms
Azides Circular Dichroism Hydrogen-Ion Concentration Kinetics Nucleic Acid Conformation Poly A/analogs & derivatives,chemical synthesis Polydeoxyribonucleotides/chemical synthesis Spectrophotometry, Ultraviolet Temperature
Chemicals
Azides Polydeoxyribonucleotides Poly A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ikehara M
Fukui T
Kakiuchi N
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16 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/3.8.2089
Published
1976-08-00
Pages
2089-99
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343064
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/3.8.2089 PMC Full Text View journal details
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