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PMID: 9630217 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of a gene encoding a hyperpolarization-activated pacemaker channel of brain.

Cell ·Vol. 93 ·No. 5 ·1998-05-29 ·Pages 717-29

Santoro B, Liu DT, Yao H, Bartsch D, Kandel ER, Siegelbaum SA, Tibbs GR

Abstract

The generation of pacemaker activity in heart and brain is mediated by hyperpolarization-activated cation channels that are directly regulated by cyclic nucleotides. We previously cloned a novel member of the voltage-gated K channel family from mouse brain (mBCNG-1) that contained a carboxy-terminal cyclic nucleotide-binding domain (Santoro et al., 1997) and hence proposed it to be a candidate gene for pacemaker channels. Heterologous expression of mBCNG-1 demonstrates that it does indeed code for a channel with properties indistinguishable from pacemaker channels in brain and similar to those in heart. Three additional mouse genes and two human genes closely related to mBCNG-1 display unique patterns of mRNA expression in different tissues, including brain and heart, demonstrating that these channels constitute a widely expressed gene family.

MeSH Terms

Amino Acid Sequence Animals Barium/pharmacology Biological Clocks/genetics Brain/physiology Cesium/pharmacology Cloning, Molecular Cyclic AMP/pharmacology Cyclic Nucleotide-Gated Cation Channels DNA, Complementary/genetics Electric Conductivity Gene Expression Heart/physiology Humans Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels Ion Channels/antagonists & inhibitors,biosynthesis,genetics Mice Molecular Sequence Data Multigene Family Nerve Tissue Proteins/antagonists & inhibitors,biosynthesis,genetics Neuroglia/metabolism Oocytes Pacemaker, Artificial Patch-Clamp Techniques Potassium/metabolism Potassium Channels Recombinant Proteins/biosynthesis Sequence Homology, Amino Acid Sodium/metabolism Species Specificity Tissue Distribution Xenopus

Chemicals

Cyclic Nucleotide-Gated Cation Channels DNA, Complementary Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels Ion Channels Nerve Tissue Proteins Potassium Channels Recombinant Proteins Cesium Barium Sodium Cyclic AMP Potassium

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Santoro B

Center for Neurobiology and Behavior, Columbia University, New York, New York 10032, USA.

Liu D T

Yao H

Bartsch D

Kandel E R

Siegelbaum S A

Tibbs G R

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/s0092-8674(00)81434-8

Published

1998-05-29

Pages

717-29

Language

English

Region

United States

NLM ID

0413066

Subset

Grants

NINDS NIH HHS · R01 NS 36658-01A1 · United States

Databases

GENBANK

AF064873, AF064874, AF064875, AF064876, AF064877

External Links

PubMed source DOI: 10.1016/s0092-8674(00)81434-8 View journal details

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Identification of a gene encoding a hyperpolarization-activated pacemaker channel of brain.

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