Erythroid colony formation (CFUe) in fetal liver and adult bone marrow and spleen from the mouse.

Blut ·Vol. 33 ·No. 3 ·1976-09-00 ·Pages 171-80

Rich IN, Kubanek B

Abstract

The methyl cellulose modification of the CFUe technique has been applied to 14 day fetal liver and adult bone marrow and spleen from CBA/CA mice. Optimized doses of fetal calf serum, alpha-thioglycerol, erythropoietin and cell suspensions have been obtained from dose response curves in order to standardize the technique. The slopes of the erythropoietin and cell dose response curves indicate a greater sensitivity by fetal liver to the hormone than bone marrow or spleen. The proportion of cells in the DNA synthesis phase of the cell cycle, as measured by the CFUe technique, has been estimated by administering hydroxyurea. Two hours after the drug was injected, 89% of fetal liver cells, 71% of bone marrow cells and 81% of spleen cells were found to be in the S-phase.

MeSH Terms

Animals Bone Marrow/drug effects Bone Marrow Cells Cattle Cell Division/drug effects Dose-Response Relationship, Drug Erythropoiesis/drug effects Erythropoietin/pharmacology Female Glycerol/analogs & derivatives,pharmacology Hydroxyurea/pharmacology Liver/enzymology Methylcellulose/pharmacology Mice Mice, Inbred CBA Pregnancy Spleen/drug effects

Chemicals

Erythropoietin Methylcellulose Glycerol Hydroxyurea

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Rich I N

Kubanek B

References (26)

26 references, click to expand

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Article Info

Journal

Blut

Abbr.

Blut

ISSN

0006-5242

DOI

10.1007/BF00995998

Published

1976-09-00

Pages

171-80

Language

English

Region

Germany

NLM ID

0173401

Subset

External Links

PubMed source DOI: 10.1007/BF00995998

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Erythroid colony formation (CFUe) in fetal liver and adult bone marrow and spleen from the mouse.

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