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PMID: 9640 Published · ppublish English Journal Article

Supersaturation in sickle cell hemoglobin solutions.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 9 ·1976-09-00 ·Pages 3035-9

Hofrichter J, Ross PD, Eaton WA

Abstract

The kinetic inhibition of the gelation of hemoglobin S is compared to the change in hemoglobin S soulbility, when the solubility is altered by carbon monoxide, pH, or urea. By means of a new technique, the delay time and the extent of gelation are measured on the same sample. They delay time, td, is found to be proportional to a high power (30-40) of the hemoglobin S solubility. Togehter with the previously reported concentration dependence, this result demonstrates that the rate is proportional to a high power of the supersaturation, S, defined as the ratio of the total hemoglobin S concentration to the equilibrium solubility. The results obey the supersaturation equation td-1 = gammaSn, where gamma is an empirical constant (about 10(-7) sec-1) and n is about 35. The supersaturation equation can successfully account for observations on the kinetics of cell sickling and is therefore used to estimate the increase in the delay time for sickling necessary to produce significant clinical benefit to patients with sickle cell disease.

MeSH Terms
Carbon Monoxide Gels Humans Hydrogen-Ion Concentration Kinetics Solubility Thermodynamics Urea
Chemicals
Gels Carbon Monoxide Urea
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hofrichter J
Ross P D
Eaton W A
References (24)
24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.9.3035
Published
1976-09-00
Pages
3035-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430918
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.9.3035 PMC Full Text View journal details
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