Molecular epidemiology of reemergent Vibrio cholerae O139 Bengal in India.

Journal of clinical microbiology ·Vol. 36 ·No. 7 ·1998-07-00 ·Pages 2149-52

Mukhopadhyay AK, Basu A, Garg P, Bag PK, Ghosh A, Bhattacharya SK, Takeda Y, Nair GB

Abstract

We report the prevalence of the O139 serogroup in Calcutta, India, after its reemergence in August 1996 and the spread of the reemerged clone to other parts of the country by using previously established molecular markers. Phenotypically, the reemerged Vibrio cholerae O139 displayed a difference compared to those that appeared in late 1992 and 1993 in that the current O139 strains are sensitive to co-trimoxazole. Ribotyping with the enzyme BglI produced two rRNA restriction patterns in the O139 strains isolated after August 1996, and these patterns were identical to those exhibited by strains of O139 isolated in 1992. Three clones of V. cholerae O139 are currently prevailing in the country, with strains exhibiting three bands after HindIII digestion and hybridization with a ctxA probe being dominant. The reemergence of V. cholerae O139 in Calcutta after a 32-month quiescent period reestablishes the O139 serogroup as an entity which is likely to play a crucial role in the temporal antigenic variations among the serogroups of V. cholerae causing cholera.

MeSH Terms

Bacterial Typing Techniques Cholera/epidemiology,microbiology Feces/microbiology Humans India/epidemiology Molecular Epidemiology Polymorphism, Restriction Fragment Length RNA, Bacterial/genetics RNA, Ribosomal/genetics Restriction Mapping Seasons Serotyping Vibrio cholerae/classification,genetics,isolation & purification

Chemicals

RNA, Bacterial RNA, Ribosomal

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Mukhopadhyay A K

National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.

Basu A

Garg P

Bag P K

Ghosh A

Bhattacharya S K

Takeda Y

Nair G B

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21 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/JCM.36.7.2149-2152.1998

Published

1998-07-00

Pages

2149-52

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC105015

Subset

External Links

PubMed source DOI: 10.1128/JCM.36.7.2149-2152.1998 PMC Full Text View journal details

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Molecular epidemiology of reemergent Vibrio cholerae O139 Bengal in India.

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