Home LiteratureArticle Details
PMID: 9651219 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Type 1 inositol 1,4,5-trisphosphate receptor is required for induction of long-term depression in cerebellar Purkinje neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 18 ·No. 14 ·1998-07-15 ·Pages 5366-73

Inoue T, Kato K, Kohda K, Mikoshiba K

Abstract

The inositol 1,4,5-trisphosphate receptor (InsP3R) is an intracellular Ca2+ channel that releases Ca2+ from internal Ca2+ stores in response to InsP3. Although InsP3R is highly expressed in various regions of the mammalian brain, the functional role of this receptor has not been clarified. We show here that cerebellar slices prepared from mice with a disrupted InsP3R type 1 gene, which is predominantly expressed in Purkinje cells, completely lack long-term depression (LTD), a model of synaptic plasticity in the cerebellum. Moreover, a specific antibody against InsP3R1, introduced into wild-type Purkinje cells through patch pipettes, blocked the induction of LTD. These data indicate that, in addition to Ca2+ influx through Ca2+ channels on the plasma membrane, Ca2+ release from InsP3R plays an essential role in the induction of LTD, suggesting a physiological importance for InsP3R in Purkinje cells.

MeSH Terms
Animals Antibodies, Monoclonal Antibody Specificity Calcium Channels/physiology In Vitro Techniques Inositol 1,4,5-Trisphosphate Inositol 1,4,5-Trisphosphate Receptors Mice Mice, Neurologic Mutants Neuronal Plasticity/physiology Patch-Clamp Techniques Purkinje Cells/physiology Receptors, Cytoplasmic and Nuclear/physiology
Chemicals
Antibodies, Monoclonal Calcium Channels Inositol 1,4,5-Trisphosphate Receptors Receptors, Cytoplasmic and Nuclear Inositol 1,4,5-Trisphosphate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Inoue T
Department of Molecular Neurobiology, The Institute of Medical Science, The University of Tokyo, Tokyo-108, Japan.
Kato K
Kohda K
Mikoshiba K
References (41)
41 references, click to expand
  1. Xestospongins: potent membrane permeable blockers of the inositol 1,4,5-trisphosphate receptor.
    Neuron. 1997 Sep;19(3):723-33 PMID: 9331361
  2. Two types of ryanodine receptors in mouse brain: skeletal muscle type exclusively in Purkinje cells and cardiac muscle type in various neurons.
    Neuron. 1992 Dec;9(6):1133-42 PMID: 1334420
  3. Intradendritic release of calcium induced by glutamate in cerebellar Purkinje cells.
    Neuron. 1991 Oct;7(4):577-83 PMID: 1681831
  4. Spatially resolved calcium dynamics of mammalian Purkinje cells in cerebellar slice.
    Science. 1988 Nov 4;242(4879):773-7 PMID: 2847315
  5. Trans-ACPD-induced Ca2+ signals in cerebellar Purkinje cells.
    Neuroreport. 1991 Dec;2(12):759-62 PMID: 1793818
  6. Activation of Ca(2+)-dependent K+ current by acetylcholine and histamine in a human gastric epithelial cell line.
    J Gen Physiol. 1993 Oct;102(4):667-92 PMID: 8270909
  7. Block of Ca2+ wave and Ca2+ oscillation by antibody to the inositol 1,4,5-trisphosphate receptor in fertilized hamster eggs.
    Science. 1992 Jul 10;257(5067):251-5 PMID: 1321497
  8. Receptors and second messengers involved in long-term depression in rat cerebellar slices in vitro: a reappraisal.
    Eur J Neurosci. 1995 Jan 1;7(1):45-53 PMID: 7711936
  9. Activity patterns of cerebellar cortical neurones and climbing fibre afferents in the awake cat.
    J Physiol. 1979 Apr;289:425-48 PMID: 458677
  10. Long-term synaptic depression in the mammalian brain.
    Neuron. 1994 Mar;12(3):457-72 PMID: 8155315
  11. Ca2+ release from Ca2+ stores, particularly from ryanodine-sensitive Ca2+ stores, is required for the induction of LTD in cultured cerebellar Purkinje cells.
    J Neurophysiol. 1995 Nov;74(5):2184-8 PMID: 8592207
  12. Range of messenger action of calcium ion and inositol 1,4,5-trisphosphate.
    Science. 1992 Dec 11;258(5089):1812-5 PMID: 1465619
  13. Brief dendritic calcium signals initiate long-lasting synaptic depression in cerebellar Purkinje cells.
    Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7051-5 PMID: 1323125
  14. Induction of cerebellar long-term depression requires activation of glutamate metabotropic receptors.
    Neuroreport. 1994 Apr 14;5(8):913-6 PMID: 8061295
  15. Antibodies inactivating mGluR1 metabotropic glutamate receptor block long-term depression in cultured Purkinje cells.
    Neuron. 1994 Jun;12(6):1245-55 PMID: 7912091
  16. Identification and localization of ryanodine binding proteins in the avian central nervous system.
    Neuron. 1990 Aug;5(2):135-46 PMID: 2200448
  17. Long-term depression.
    Annu Rev Neurosci. 1989;12:85-102 PMID: 2648961
  18. Deficient cerebellar long-term depression and impaired motor learning in mGluR1 mutant mice.
    Cell. 1994 Oct 21;79(2):377-88 PMID: 7954803
  19. Functional heterogeneity of calcium release by inositol trisphosphate in single Purkinje neurones, cultured cerebellar astrocytes, and peripheral tissues.
    Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):4976-80 PMID: 8506344
  20. Fast activation and inactivation of inositol trisphosphate-evoked Ca2+ release in rat cerebellar Purkinje neurones.
    J Physiol. 1995 Sep 1;487 ( Pt 2):343-58 PMID: 8558468
  21. Widespread expression of inositol 1,4,5-trisphosphate receptor type 1 gene (Insp3r1) in the mouse central nervous system.
    Receptors Channels. 1993;1(1):11-24 PMID: 8081710
  22. Involvement of the C-terminus of the inositol 1,4,5-trisphosphate receptor in Ca2+ release analysed using region-specific monoclonal antibodies.
    Biochem J. 1991 Jul 1;277 ( Pt 1):125-31 PMID: 1713032
  23. Calcium transients in cerebellar Purkinje neurons evoked by intracellular stimulation.
    J Neurophysiol. 1992 Oct;68(4):1167-77 PMID: 1432076
  24. Ataxia and epileptic seizures in mice lacking type 1 inositol 1,4,5-trisphosphate receptor.
    Nature. 1996 Jan 11;379(6561):168-71 PMID: 8538767
  25. Electrophysiological properties of in vitro Purkinje cell somata in mammalian cerebellar slices.
    J Physiol. 1980 Aug;305:171-95 PMID: 7441552
  26. Impaired motor coordination correlates with persistent multiple climbing fiber innervation in PKC gamma mutant mice.
    Cell. 1995 Dec 29;83(7):1233-42 PMID: 8548809
  27. Participation of postsynaptic PKC in cerebellar long-term depression in culture.
    Science. 1991 Dec 13;254(5038):1656-9 PMID: 1721243
  28. Involvement of inositol trisphosphate in cerebellar long-term depression.
    Neuroreport. 1995 Feb 15;6(3):569-72 PMID: 7539306
  29. Inositol trisphosphate and calcium signalling.
    Nature. 1993 Jan 28;361(6410):315-25 PMID: 8381210
  30. Calcium is an intracellular mediator of the climbing fiber in induction of cerebellar long-term depression.
    Proc Natl Acad Sci U S A. 1990 May;87(9):3383-5 PMID: 2159149
  31. Effect of pentosan polysulphate, standard heparin and related compounds on protein kinase C activity.
    Biochim Biophys Acta. 1991 Feb 19;1091(3):432-41 PMID: 1705825
  32. Activation of protein kinase C induces a long-term depression of glutamate sensitivity of cerebellar Purkinje cells. An in vitro study.
    Brain Res. 1988 Aug 23;458(2):397-401 PMID: 2905201
  33. Spatial dynamics of second messengers: IP3 and cAMP as long-range and associative messengers.
    Trends Neurosci. 1994 Mar;17(3):95-101 PMID: 7515531
  34. Primary structure and functional expression of the inositol 1,4,5-trisphosphate-binding protein P400.
    Nature. 1989 Nov 2;342(6245):32-8 PMID: 2554142
  35. Bell-shaped calcium-response curves of Ins(1,4,5)P3- and calcium-gated channels from endoplasmic reticulum of cerebellum.
    Nature. 1991 Jun 27;351(6329):751-4 PMID: 1648178
  36. cGMP acts within cerebellar Purkinje cells to produce long term depression via mechanisms involving PKC and PKG.
    Neuroreport. 1994 Mar 21;5(7):833-6 PMID: 7517198
  37. Calcium-induced calcium release in cerebellar Purkinje cells.
    Neuron. 1994 Mar;12(3):663-73 PMID: 7512352
  38. Calcium transients evoked by climbing fiber and parallel fiber synaptic inputs in guinea pig cerebellar Purkinje neurons.
    J Neurophysiol. 1992 Oct;68(4):1178-89 PMID: 1359027
  39. Motor deficit and impairment of synaptic plasticity in mice lacking mGluR1.
    Nature. 1994 Nov 17;372(6503):237-43 PMID: 7969468
  40. Pairing of pre- and postsynaptic activities in cerebellar Purkinje cells induces long-term changes in synaptic efficacy in vitro.
    J Physiol. 1991 Jan;432:123-41 PMID: 1886056
  41. Induction of long-term depression and rebound potentiation by inositol trisphosphate in cerebellar Purkinje neurons.
    Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):14009-14 PMID: 9391143
Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1998-07-15
Pages
5366-73
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6793478
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2