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PMID: 967676 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Diversity of sequences in total and polyadenylated nuclear RNA from Drosophila cells.

Nucleic acids research ·Vol. 3 ·No. 7 ·1976-07-00 ·Pages 1777-89

Levy B, Johnson CB, McCarthy BJ

Abstract

Complementary DNA was synthesized using polyadenylated nuclear RNA of cultured Drosophila cells as template. The kinetics of hybridization of this cDNA with nuclear RNA indicated that the complexity of this RNA population is five to ten times greater than that of cytoplasmic mRNA. The same difference in the fraction of DNA represented was obtained when nuclear and cytoplasmic RNA were hybridized with labeled unique sequence DNA. The fraction of the DNA sequences represented in total number of polyadenylated nuclear RNA is much higher than that represented in cytoplasmic RNA.

MeSH Terms
Base Sequence Cell Line Cell Nucleus/analysis Drosophila Nucleic Acid Hybridization Nucleic Acid Renaturation Poly A/analysis RNA/isolation & purification
Chemicals
Poly A RNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Levy B
Johnson C B
McCarthy B J
References (20)
20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/3.7.1777
Published
1976-07-00
Pages
1777-89
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343036
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/3.7.1777 PMC Full Text View journal details
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