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PMID: 9679148 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A-kinase anchoring protein 100 (AKAP100) is localized in multiple subcellular compartments in the adult rat heart.

The Journal of cell biology ·Vol. 142 ·No. 2 ·1998-07-27 ·Pages 511-22

Yang J, Drazba JA, Ferguson DG, Bond M

Abstract

Stimulation of beta-adrenergic receptors activates type I and II cyclic AMP-dependent protein kinase A, resulting in phosphorylation of various proteins in the heart. It has been proposed that PKA II compartmentalization by A-kinase-anchoring proteins (AKAPs) regulates cyclic AMP-dependent signaling in the cell. We investigated the expression and localization of AKAP100 in adult hearts. By immunoblotting, we identified AKAP100 in adult rat and human hearts, and showed that type I and II regulatory (RI and II) subunits of PKA are present in the rat heart. By immunofluorescence and confocal microscopy of rat cardiac myocytes and cryostat sections of rat left ventricle papillary muscles, we localized AKAP100 to the nucleus, sarcolemma, intercalated disc, and at the level of the Z-line. After double immunostaining of transverse cross-sections of the papillary muscles with AKAP100 plus alpha-actinin-specific antibodies or AKAP100 plus ryanodine receptor-specific antibodies, confocal images showed AKAP100 localization at the region of the transverse tubule/junctional sarcoplasmic reticulum. RI is distributed differently from RII in the myocytes. RII, but not RI, was colocalized with AKAP100 in the rat heart. Our studies suggest that AKAP100 tethers PKA II to multiple subcellular compartments for phosphorylation of different pools of substrate proteins in the heart.

MeSH Terms
A Kinase Anchor Proteins Adaptor Proteins, Signal Transducing Adult Animals Antibody Specificity Base Sequence Carrier Proteins Cyclic AMP-Dependent Protein Kinases/chemistry,immunology,metabolism DNA Primers/genetics Humans Immunohistochemistry Myocardium/metabolism,ultrastructure Phosphorylation Proteins/genetics,immunology,metabolism Rats Rats, Sprague-Dawley Recombinant Proteins/genetics,immunology,metabolism Sarcoplasmic Reticulum/metabolism Subcellular Fractions/metabolism
Chemicals
A Kinase Anchor Proteins AKAP6 protein, human Adaptor Proteins, Signal Transducing Akap6 protein, rat Carrier Proteins DNA Primers Proteins Recombinant Proteins Cyclic AMP-Dependent Protein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yang J
Department of Molecular Cardiology, The Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
Drazba J A
Ferguson D G
Bond M
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.142.2.511
Published
1998-07-27
Pages
511-22
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2133049
Subset
IM
Grants
NHLBI NIH HHS · HL07653 · United States
NHLBI NIH HHS · R01 HL56256 · United States
External Links
PubMed source DOI: 10.1083/jcb.142.2.511 PMC Full Text View journal details
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