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PMID: 9693380 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cysteine string protein functions directly in regulated exocytosis.

Molecular biology of the cell ·Vol. 9 ·No. 8 ·1998-08-00 ·Pages 2259-67

Chamberlain LH, Burgoyne RD

Abstract

Cysteine string protein (Csp) is essential for neurotransmitter release in Drosophila. It has been suggested that Csp functions by regulating the activity of presynaptic Ca2+ channels, thus controlling exocytosis. We have examined the effect of overexpressing Csp1 in PC12 cells, a neuroendocrine cell line. PC12 cell clones overexpressing Csp1 did not show any changes in morphology, granule number or distribution, or in the levels of other key exocytotic proteins. This overexpression did not affect intracellular Ca2+ signals after depolarization, suggesting that Csp1 has no gross effect on Ca2+ channel activity in PC12 cells. In contrast, we show that Csp1 overexpression enhances the extent of exocytosis from permeabilized cells in response to Ca2+ or GTPgammaS in the absence of Ca2+. Because secretion from permeabilized cells is not influenced by Ca2+ channel activity, this represents the first demonstration that Csp has a direct role in regulated exocytosis.

MeSH Terms
Animals Calcium/metabolism Cell Fractionation Cell Membrane/metabolism,ultrastructure Clone Cells Dopamine/metabolism Exocytosis HSP40 Heat-Shock Proteins Homeostasis Kinetics Membrane Proteins Nerve Tissue Proteins/biosynthesis,metabolism PC12 Cells Rats Recombinant Proteins/biosynthesis,metabolism Signal Transduction Transfection
Chemicals
HSP40 Heat-Shock Proteins Membrane Proteins Nerve Tissue Proteins Recombinant Proteins cysteine string protein Calcium Dopamine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chamberlain L H
The Physiological Laboratory, Liverpool University, Liverpool L69 3BX, United Kingdom.
Burgoyne R D
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.9.8.2259
Published
1998-08-00
Pages
2259-67
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25479
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1091/mbc.9.8.2259 PMC Full Text View journal details
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