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PMID: 9694792 Published · ppublish English Journal Article

A new NEDD8-ligating system for cullin-4A.

Genes & development ·Vol. 12 ·No. 15 ·1998-08-01 ·Pages 2263-8

Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S

Abstract

NEDD8 is a ubiquitin (Ub)-like protein. Here we report a novel ubiquitinylation-related pathway for modification by NEDD8. NEDD8 was activated by an E1 (Ub-activating enzyme)-like complex, consisting of APP-BP1 and hUba3 with high respective homologies to the amino- and carboxy-terminal regions of E1 and then linked to hUbc12 (a human homolog of yeast Ub-conjugating enzyme Ubc12p). The major target protein modified by NEDD8 was found to be Hs-cullin-4A (Cul-4A), a member of the family of human cullin/Cdc53 proteins functioning as an essential component of a multifunctional Ub-protein ligase E3 complex that has a critical role in Ub-mediated proteolysis.

MeSH Terms
Amino Acid Sequence Animals Cell Cycle Proteins/genetics,metabolism Cullin Proteins DNA-Binding Proteins/genetics,metabolism Humans In Vitro Techniques Ligases/genetics,metabolism Molecular Sequence Data NEDD8 Protein Rabbits Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Ubiquitin-Activating Enzymes Ubiquitin-Protein Ligases Ubiquitins/genetics,metabolism
Chemicals
Cell Cycle Proteins Cullin 1 Cullin Proteins DNA-Binding Proteins NEDD8 Protein NEDD8 protein, human Saccharomyces cerevisiae Proteins UBC12 protein, S cerevisiae Ubiquitins Ubiquitin-Protein Ligases Ligases Ubiquitin-Activating Enzymes NAE protein, human
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Osaka F
Kato Cytoprotein Network Project, ERATO, Japan Science and Technology Corporation (JST), Sagami Chemical Research Center, Sagamihara, Kanagawa 229-0012, Japan.
Kawasaki H
Aida N
Saeki M
Chiba T
Kawashima S
Tanaka K
Kato S
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.15.2263
Published
1998-08-01
Pages
2263-8
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC317039
Subset
IM
Databases
GENBANK
AB012190, AB012191, AB012193, D23662
External Links
PubMed source DOI: 10.1101/gad.12.15.2263 PMC Full Text View journal details
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