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PMID: 9696878 Published · ppublish English Journal Article

Characterization of unusual escape variants of hepatitis B virus isolated from a hepatitis B surface antigen-negative subject.

Journal of virology ·Vol. 72 ·No. 9 ·1998-09-00 ·Pages 7692-6

Grethe S, Monazahian M, Böhme I, Thomssen R

Abstract

Hepatitis B virus DNA was extracted from serial serum samples of a hepatitis B surface antigen-negative patient with antibodies to the core protein as the only marker of an infection with hepatitis B virus. This patient showed no symptoms of hepatic injury. Sequencing of the amplified viral DNA demonstrated multiple amino acid changes clustering in surface-exposed regions of the surface protein. Synthesis and association of the middle (M) and small (S) surface proteins could be shown in vitro. The variant surface antigens were recognized neither by monoclonal antibodies to the surface antigen nor by the vaccinee's sera. Consequences for hepatitis B surface antigen testing and vaccine development are discussed.

MeSH Terms
Amino Acid Sequence Animals Base Sequence COS Cells DNA, Viral Genetic Variation Hepatitis B/virology Hepatitis B Surface Antigens/genetics Hepatitis B virus/classification,genetics,isolation & purification Humans Male Molecular Sequence Data Protein Precursors/genetics
Chemicals
DNA, Viral Hepatitis B Surface Antigens Protein Precursors presurface protein 2, hepatitis B surface antigen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Grethe S
Department of Medical Microbiology, University of Göttingen, D-37075 Göttingen, Germany. [email protected]
Monazahian M
Böhme I
Thomssen R
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.72.9.7692-7696.1998
Email
[email protected]
Published
1998-09-00
Pages
7692-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC110046
Subset
IM
Databases
GENBANK
AJ003026, AJ003027, AJ003028, AJ003116
External Links
PubMed source DOI: 10.1128/JVI.72.9.7692-7696.1998 PMC Full Text View journal details
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