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PMID: 9701588 Published · ppublish English Journal Article

Expressed sequence tags from a root-hair-enriched medicago truncatula cDNA library

Plant physiology ·Vol. 117 ·No. 4 ·1998-08-00 ·Pages 1325-32

Covitz PA, Smith LS, Long SR

Abstract

The root hair is a specialized cell type involved in water and nutrient uptake in plants. In legumes the root hair is also the primary site of recognition and infection by symbiotic nitrogen-fixing Rhizobium bacteria. We have studied the root hairs of Medicago truncatula, which is emerging as an increasingly important model legume for studies of symbiotic nodulation. However, only 27 genes from M. truncatula were represented in GenBank/EMBL as of October, 1997. We report here the construction of a root-hair-enriched cDNA library and single-pass sequencing of randomly selected clones. Expressed sequence tags (899 total, 603 of which have homology to known genes) were generated and made available on the Internet. We believe that the database and the associated DNA materials will provide a useful resource to the community of scientists studying the biology of roots, root tips, root hairs, and nodulation.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Covitz
Department of Biological Sciences (P.A.C., L.S.S., S.R.L.).
Smith
Long
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.117.4.1325
Published
1998-08-00
Pages
1325-32
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC34896
External Links
PubMed source DOI: 10.1104/pp.117.4.1325 PMC Full Text View journal details
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