Home LiteratureArticle Details
PMID: 9707440 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Zuotin, a ribosome-associated DnaJ molecular chaperone.

The EMBO journal ·Vol. 17 ·No. 16 ·1998-08-17 ·Pages 4809-17

Yan W, Schilke B, Pfund C, Walter W, Kim S, Craig EA

Abstract

Correct folding of newly synthesized polypeptides is thought to be facilitated by Hsp70 molecular chaperones in conjunction with DnaJ cohort proteins. In Saccharomyces cerevisiae, SSB proteins are ribosome-associated Hsp70s which interact with the newly synthesized nascent polypeptide chain. Here we report that the phenotypes of an S.cerevisiae strain lacking the DnaJ-related protein Zuotin (Zuo1) are very similar to those of a strain lacking Ssb, including sensitivities to low temperatures, certain protein synthesis inhibitors and high osmolarity. Zuo1, which has been shown previously to be a nucleic acid-binding protein, is also a ribosome-associated protein localized predominantly in the cytosol. Analysis of zuo1 deletion and truncation mutants revealed a positive correlation between the ribosome association of Zuo1 and its ability to bind RNA. We propose that Zuo1 binds to ribosomes, in part, by interaction with ribosomal RNA and that Zuo1 functions with Ssb as a chaperone on the ribosome.

MeSH Terms
Cytosol/metabolism DNA-Binding Proteins/metabolism Fungal Proteins/metabolism HSP40 Heat-Shock Proteins HSP70 Heat-Shock Proteins/metabolism Heat-Shock Proteins/metabolism Molecular Chaperones Phenotype Ribosomes/metabolism Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins
Chemicals
DNA-Binding Proteins Fungal Proteins HSP40 Heat-Shock Proteins HSP70 Heat-Shock Proteins Heat-Shock Proteins Molecular Chaperones SSB1 protein, S cerevisiae Saccharomyces cerevisiae Proteins ZUO1 protein, S cerevisiae
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Yan W
Department of Biomolecular Chemistry, University of Wisconsin, 1300 University Avenue, Madison, WI 53706, USA.
Schilke B
Pfund C
Walter W
Kim S
Craig E A
References (40)
40 references, click to expand
  1. The NH2-terminal 108 amino acids of the Escherichia coli DnaJ protein stimulate the ATPase activity of DnaK and are sufficient for lambda replication.
    J Biol Chem. 1994 Feb 18;269(7):5446-51 PMID: 8106526
  2. Kinetics of molecular chaperone action.
    Science. 1994 Feb 18;263(5149):971-3 PMID: 8310296
  3. Transfer RNA binding protein in the nucleus of Saccharomyces cerevisiae.
    FEBS Lett. 1994 Aug 1;349(2):260-4 PMID: 8050578
  4. Interaction sites of ribosome-bound eukaryotic elongation factor 2 in 18S and 28S rRNA.
    Biochemistry. 1994 Dec 20;33(50):15159-67 PMID: 7999776
  5. Purified yeast translational initiation factor eIF-3 is an RNA-binding protein complex that contains the PRT1 protein.
    J Biol Chem. 1994 Dec 23;269(51):32286-92 PMID: 7798228
  6. The conserved G/F motif of the DnaJ chaperone is necessary for the activation of the substrate binding properties of the DnaK chaperone.
    J Biol Chem. 1995 Feb 3;270(5):2139-44 PMID: 7836443
  7. Modulation of the ATPase activity of the molecular chaperone DnaK by peptides and the DnaJ and GrpE heat shock proteins.
    J Biol Chem. 1995 Mar 3;270(9):4563-9 PMID: 7876226
  8. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds.
    Gene. 1995 Apr 14;156(1):119-22 PMID: 7737504
  9. Protein-RNA recognition.
    Annu Rev Biochem. 1995;64:593-620 PMID: 7574494
  10. Role of auxilin in uncoating clathrin-coated vesicles.
    Nature. 1995 Dec 7;378(6557):632-5 PMID: 8524399
  11. A cycle of binding and release of the DnaK, DnaJ and GrpE chaperones regulates activity of the Escherichia coli heat shock transcription factor sigma32.
    EMBO J. 1996 Feb 1;15(3):607-17 PMID: 8599944
  12. A zinc finger-like domain of the molecular chaperone DnaJ is involved in binding to denatured protein substrates.
    EMBO J. 1996 Jan 15;15(2):408-17 PMID: 8617216
  13. Escherichia coli trigger factor is a prolyl isomerase that associates with nascent polypeptide chains.
    Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4437-41 PMID: 8633085
  14. A bipartite signaling mechanism involved in DnaJ-mediated activation of the Escherichia coli DnaK protein.
    J Biol Chem. 1996 May 10;271(19):11236-46 PMID: 8626673
  15. Molecular chaperones in cellular protein folding.
    Nature. 1996 Jun 13;381(6583):571-9 PMID: 8637592
  16. Functional interaction of cytosolic hsp70 and a DnaJ-related protein, Ydj1p, in protein translocation in vivo.
    Mol Cell Biol. 1996 Aug;16(8):4378-86 PMID: 8754838
  17. The nascent polypeptide-associated complex modulates interactions between the signal recognition particle and the ribosome.
    Curr Biol. 1996 Mar 1;6(3):331-8 PMID: 8805251
  18. Green fluorescent protein as a marker for gene expression and subcellular localization in budding yeast.
    Yeast. 1996 Jun 30;12(8):773-86 PMID: 8813764
  19. Binding of an N-terminal rhodanese peptide to DnaJ and to ribosomes.
    J Biol Chem. 1996 Dec 6;271(49):31160-5 PMID: 8940114
  20. The lumenal domain of Sec63p stimulates the ATPase activity of BiP and mediates BiP recruitment to the translocon in Saccharomyces cerevisiae.
    J Cell Biol. 1997 Jun 30;137(7):1483-93 PMID: 9199165
  21. Protein folding in vivo: unraveling complex pathways.
    Cell. 1997 Jul 25;90(2):201-4 PMID: 9244293
  22. The Hsp70 and Hsp60 chaperone machines.
    Cell. 1998 Feb 6;92(3):351-66 PMID: 9476895
  23. The molecular chaperone Ssb from Saccharomyces cerevisiae is a component of the ribosome-nascent chain complex.
    EMBO J. 1998 Jul 15;17(14):3981-9 PMID: 9670014
  24. A mutant of yeast apparently defective in the initiation of protein synthesis.
    Proc Natl Acad Sci U S A. 1969 Feb;62(2):468-74 PMID: 5256225
  25. Analysis of temperature-sensitive mutant ts 187 of Saccharomyces cerevisiae altered in a component required for the initiation of protein synthesis.
    J Biol Chem. 1982 Sep 25;257(18):10846-51 PMID: 7050121
  26. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  27. Identification and characterization of a new Escherichia coli gene that is a dosage-dependent suppressor of a dnaK deletion mutation.
    J Bacteriol. 1990 Apr;172(4):2055-64 PMID: 2180916
  28. Isolation and characterization of dnaJ null mutants of Escherichia coli.
    J Bacteriol. 1990 Sep;172(9):4827-35 PMID: 2144273
  29. Escherichia coli DnaJ and GrpE heat shock proteins jointly stimulate ATPase activity of DnaK.
    Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2874-8 PMID: 1826368
  30. Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase.
    Anal Biochem. 1991 Feb 1;192(2):262-7 PMID: 1852137
  31. MAS5, a yeast homolog of DnaJ involved in mitochondrial protein import.
    Mol Cell Biol. 1992 Jan;12(1):283-91 PMID: 1729605
  32. Physical interaction between heat shock proteins DnaK, DnaJ, and GrpE and the bacterial heat shock transcription factor sigma 32.
    Cell. 1992 May 29;69(5):833-42 PMID: 1534276
  33. The translation machinery and 70 kd heat shock protein cooperate in protein synthesis.
    Cell. 1992 Oct 2;71(1):97-105 PMID: 1394434
  34. Zuotin, a putative Z-DNA binding protein in Saccharomyces cerevisiae.
    EMBO J. 1992 Oct;11(10):3787-96 PMID: 1396572
  35. YDJ1p facilitates polypeptide translocation across different intracellular membranes by a conserved mechanism.
    Cell. 1992 Dec 24;71(7):1143-55 PMID: 1473150
  36. RNA recognition: a family matter?
    Cell. 1993 Jun 4;73(5):837-40 PMID: 8500177
  37. The yeast SIS1 protein, a DnaJ homolog, is required for the initiation of translation.
    Cell. 1993 Jun 18;73(6):1175-86 PMID: 8513501
  38. Control of folding and membrane translocation by binding of the chaperone DnaJ to nascent polypeptides.
    Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10216-20 PMID: 8234279
  39. A Sec63p-BiP complex from yeast is required for protein translocation in a reconstituted proteoliposome.
    J Cell Biol. 1993 Dec;123(6 Pt 1):1355-63 PMID: 8253836
  40. Differential regulation of Hsp70 subfamilies by the eukaryotic DnaJ homologue YDJ1.
    J Biol Chem. 1994 Apr 1;269(13):9798-804 PMID: 8144572
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.16.4809
Published
1998-08-17
Pages
4809-17
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170810
Subset
IM
Grants
NIGMS NIH HHS · 1F32GM17687 · United States
NIGMS NIH HHS · 5R01 GM31107 · United States
External Links
PubMed source DOI: 10.1093/emboj/17.16.4809 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2