Home LiteratureArticle Details
PMID: 9712807 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Starvation selection restores elastase and rhamnolipid production in a Pseudomonas aeruginosa quorum-sensing mutant.

Infection and immunity ·Vol. 66 ·No. 9 ·1998-09-00 ·Pages 4499-502

Van Delden C, Pesci EC, Pearson JP, Iglewski BH

Abstract

The las quorum-sensing system of Pseudomonas aeruginosa controls the expression of elastase and rhamnolipid. We report that starvation can select a mutant producing these virulence factors in spite of a lasR deletion. Expression of the autoinducer synthase gene rhlI was increased in this suppressor mutant, suggesting compensation by the rhl system. These data show that P. aeruginosa can restore elastase and rhamnolipid production in the absence of a functional las quorum-sensing system.

MeSH Terms
Bacterial Proteins/biosynthesis DNA-Binding Proteins/biosynthesis Hexosyltransferases/biosynthesis Ligases Lipids/biosynthesis Metalloendopeptidases/biosynthesis Pancreatic Elastase/biosynthesis Pseudomonas aeruginosa/metabolism Suppression, Genetic Trans-Activators/biosynthesis Transcription Factors/biosynthesis
Chemicals
Bacterial Proteins DNA-Binding Proteins LasI protein, Pseudomonas aeruginosa LasR protein, Pseudomonas aeruginosa Lipids RhlR protein, Pseudomonas aeruginosa Trans-Activators Transcription Factors Hexosyltransferases rhamnosyltransferase 1 Pancreatic Elastase Metalloendopeptidases pseudolysin, Pseudomonas aeruginosa Ligases RHLI protein, Pseudomonas aeruginosa
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Van Delden C
Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA.
Pesci E C
Pearson J P
Iglewski B H
References (25)
25 references, click to expand
  1. Chromosomal genetics of Pseudomonas.
    Microbiol Rev. 1979 Mar;43(1):73-102 PMID: 111024
  2. An RpoS (sigmaS) homologue regulates acylhomoserine lactone-dependent autoinduction in Ralstonia solanacearum.
    Mol Microbiol. 1998 May;28(3):475-86 PMID: 9632252
  3. Identification and characterization of a new Escherichia coli gene that is a dosage-dependent suppressor of a dnaK deletion mutation.
    J Bacteriol. 1990 Apr;172(4):2055-64 PMID: 2180916
  4. Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase expression.
    J Bacteriol. 1991 May;173(9):3000-9 PMID: 1902216
  5. Pseudomonas aeruginosa LasA: a second elastase under the transcriptional control of lasR.
    Mol Microbiol. 1991 Aug;5(8):2003-10 PMID: 1766376
  6. LasR of Pseudomonas aeruginosa is a transcriptional activator of the alkaline protease gene (apr) and an enhancer of exotoxin A expression.
    Infect Immun. 1993 Apr;61(4):1180-4 PMID: 8454322
  7. Microbial competition: Escherichia coli mutants that take over stationary phase cultures.
    Science. 1993 Mar 19;259(5102):1757-60 PMID: 7681219
  8. Expression of Pseudomonas aeruginosa virulence genes requires cell-to-cell communication.
    Science. 1993 May 21;260(5111):1127-30 PMID: 8493556
  9. Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes.
    Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):197-201 PMID: 8278364
  10. Sensing starvation: a homoserine lactone--dependent signaling pathway in Escherichia coli.
    Science. 1994 Jul 22;265(5171):537-9 PMID: 7545940
  11. A second N-acylhomoserine lactone signal produced by Pseudomonas aeruginosa.
    Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1490-4 PMID: 7878006
  12. Ventilator-associated pneumonia caused by Pseudomonas infection.
    Clin Chest Med. 1995 Mar;16(1):95-109 PMID: 7768096
  13. Autoinducer-mediated regulation of rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa.
    Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6424-8 PMID: 7604006
  14. Multiple homologues of LuxR and LuxI control expression of virulence determinants and secondary metabolites through quorum sensing in Pseudomonas aeruginosa PAO1.
    Mol Microbiol. 1995 Jul;17(2):333-43 PMID: 7494482
  15. Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the control of RhlR-RhlI, another set of regulators in strain PAO1 with homology to the autoinducer-responsive LuxR-LuxI family.
    J Bacteriol. 1995 Dec;177(24):7155-63 PMID: 8522523
  16. Contribution of specific Pseudomonas aeruginosa virulence factors to pathogenesis of pneumonia in a neonatal mouse model of infection.
    Infect Immun. 1996 Jan;64(1):37-43 PMID: 8557368
  17. Pulmonary infiltrates in the cancer patient. New approaches to an old problem.
    Infect Dis Clin North Am. 1996 Jun;10(2):297-325 PMID: 8803622
  18. Functional analysis of the Pseudomonas aeruginosa autoinducer PAI.
    J Bacteriol. 1996 Oct;178(20):5995-6000 PMID: 8830697
  19. Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia.
    Microbiol Rev. 1996 Sep;60(3):539-74 PMID: 8840786
  20. A hierarchical quorum-sensing cascade in Pseudomonas aeruginosa links the transcriptional activators LasR and RhIR (VsmR) to expression of the stationary-phase sigma factor RpoS.
    Mol Microbiol. 1996 Sep;21(6):1137-46 PMID: 8898383
  21. Census and consensus in bacterial ecosystems: the LuxR-LuxI family of quorum-sensing transcriptional regulators.
    Annu Rev Microbiol. 1996;50:727-51 PMID: 8905097
  22. Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa.
    J Bacteriol. 1997 May;179(10):3127-32 PMID: 9150205
  23. Contribution of proteases and LasR to the virulence of Pseudomonas aeruginosa during corneal infections.
    Infect Immun. 1997 Aug;65(8):3086-90 PMID: 9234758
  24. Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes.
    J Bacteriol. 1997 Sep;179(18):5756-67 PMID: 9294432
  25. Isolation and characterization of Pseudomonas aeruginosa PAO mutant that produces altered elastase.
    J Bacteriol. 1980 Jun;142(3):836-42 PMID: 6769912
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.66.9.4499-4502.1998
Published
1998-09-00
Pages
4499-502
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC108545
Subset
IM
Grants
NIAID NIH HHS · T32 AI007362 · United States
NIAID NIH HHS · 5-T32 AI07362-09 · United States
PHS HHS · R01A133713-04 · United States
External Links
PubMed source DOI: 10.1128/IAI.66.9.4499-4502.1998 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2