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PMID: 9724689 Published · ppublish English Journal Article

Resistance of nicotiana benthamiana to phytophthora infestans is mediated by the recognition of the elicitor protein INF1

The Plant cell ·Vol. 10 ·No. 9 ·1998-09-00 ·Pages 1413-26

Kamoun S, van West P, Vleeshouwers VG, de Groot KE, Govers F

Abstract

Phytophthora infestans, the agent of potato and tomato late blight disease, produces a 10-kD extracellular protein, INF1 elicitin. INF1 induces a hypersensitive response in a restricted number of plants, particularly those of the genus Nicotiana. In virulence assays with different P. infestans isolates, five Nicotiana species displayed resistance responses. In all of the interactions, after inoculation with P. infestans zoospores, penetration of an epidermal cell was observed, followed by localized necrosis typical of a hypersensitive response. To determine whether INF1 functions as an avirulence factor in these interactions, we adopted a gene-silencing strategy to inhibit INF1 production. Several transformants deficient in inf1 mRNA and INF1 protein were obtained. These strains remained pathogenic on host plants. However, in contrast to the wild-type and control transformant strains, INF1-deficient strains induced disease lesions when inoculated on N. benthamiana. These results demonstrate that the elicitin INF1 functions as an avirulence factor in the interaction between N. benthamiana and P. infestans.

Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kamoun
Laboratory of Phytopathology and Graduate School, Experimental Plant Sciences, Wageningen Agricultural University, Wageningen, The Netherlands.
van West P
Vleeshouwers
de Groot KE
Govers
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.10.9.1413
Published
1998-09-00
Pages
1413-26
Language
English
Region
England
NLM ID
9208688
PMCID
PMC144078
External Links
PubMed source DOI: 10.1105/tpc.10.9.1413 PMC Full Text View journal details
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