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PMID: 9724821 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A single gene of chloroplast origin codes for mitochondrial and chloroplastic methionyl-tRNA synthetase in Arabidopsis thaliana.

Menand B, Maréchal-Drouard L, Sakamoto W, Dietrich A, Wintz H

Abstract

One-fifth of the tRNAs used in plant mitochondrial translation is coded for by chloroplast-derived tRNA genes. To understand how aminoacyl-tRNA synthetases have adapted to the presence of these tRNAs in mitochondria, we have cloned an Arabidopsis thaliana cDNA coding for a methionyl-tRNA synthetase. This enzyme was chosen because chloroplast-like elongator tRNAMet genes have been described in several plant species, including A. thaliana. We demonstrate here that the isolated cDNA codes for both the chloroplastic and the mitochondrial methionyl-tRNA synthetase (MetRS). The protein is transported into isolated chloroplasts and mitochondria and is processed to its mature form in both organelles. Transient expression assays using the green fluorescent protein demonstrated that the N-terminal region of the MetRS is sufficient to address the protein to both chloroplasts and mitochondria. Moreover, characterization of MetRS activities from mitochondria and chloroplasts of pea showed that only one MetRS activity exists in each organelle and that both are indistinguishable by their behavior on ion exchange and hydrophobic chromatographies. The high degree of sequence similarity between A. thaliana and Synechocystis MetRS strongly suggests that the A. thaliana MetRS gene described here is of chloroplast origin.

MeSH Terms
Amino Acid Sequence Arabidopsis/enzymology,genetics Base Sequence Biological Transport Chloroplasts/enzymology DNA, Complementary Green Fluorescent Proteins Luminescent Proteins/genetics Methionine-tRNA Ligase/genetics,isolation & purification,metabolism Mitochondria/enzymology Molecular Sequence Data
Chemicals
DNA, Complementary Luminescent Proteins Green Fluorescent Proteins Methionine-tRNA Ligase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Menand B
Institut de Biologie Moléculaire des Plantes/Centre National de la Recherche Scientifique, Université Louis Pasteur, 12 rue du Général Zimmer, F-67084 Strasbourg Cedex, France.
Maréchal-Drouard L
Sakamoto W
Dietrich A
Wintz H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1998-09-01
Pages
11014-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC28012
Subset
IM
Databases
GENBANK
Y13943
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