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PMID: 97265 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cellular lysis of Streptococcus faecalis induced with triton X-100.

Journal of bacteriology ·Vol. 135 ·No. 1 ·1978-07-00 ·Pages 153-60

Cornett JB, Shockman GD

Abstract

Lysis of exponential-phase cultures of Streptococcus faecalis ATCC 9790 was induced by exposure to both anionic (sodium dodecyl sulfate) and nonionic (Triton X-100) surfactants. Lysis in response to sodium dodecyl sulfate was effective only over a limited range of concentrations, whereas Triton X-100-induced lysis occurred over a broad range of surfactant concentrations. The data presented indicate that the bacteriolytic response of growing cells to Triton X-100: (i) was related to the ratio of surfactant to cells and not the surfactant concentration per se; (ii) required the expression of the cellular autolytic enzyme system; and (iii) was most likely due to an effect of the surfactant on components of the autolytic system that are associated with the cytoplasmic membrane. The possibility that Triton X-100 may induce cellular lysis by releasing a lipid inhibitor of the cellular autolytic enzyme is discussed.

MeSH Terms
Bacterial Proteins/biosynthesis Bacteriolysis Cell Membrane/drug effects Chloramphenicol/pharmacology Enterococcus faecalis/drug effects,enzymology Hydrolases/metabolism Mutation Peptide Hydrolases/metabolism Polyethylene Glycols/pharmacology Quaternary Ammonium Compounds/pharmacology Sodium Dodecyl Sulfate/pharmacology
Chemicals
Bacterial Proteins Quaternary Ammonium Compounds Sodium Dodecyl Sulfate Polyethylene Glycols Chloramphenicol Hydrolases Peptide Hydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cornett J B
Shockman G D
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37 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.135.1.153-160.1978
Published
1978-07-00
Pages
153-60
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC224794
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.135.1.153-160.1978 PMC Full Text View journal details
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