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PMID: 97295 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Amino acid sequence of beta-galactosidase. VIII. Sequence of the NH2-terminal segment, CNBr peptides 1 to 9, residues 1 to 377.

The Journal of biological chemistry ·Vol. 253 ·No. 15 ·1978-08-10 ·Pages 5505-9

Fowler AV, Zabin I

Abstract

The amino acids in 9 cyanogen bromide peptides have been placed in sequence starting from the NH2 terminus. The peptides account for residues 1 to 377 of the whole protein and include the largest (CNBr7, 119 residues) and the smallest (CNBr1, 2 residues) of the cyanogen bromide peptides. This region contains only 3 of the 20 lysine residues in the polypeptide chain. A high proportion of charged groups are present (28 of 66 arginine, 28 of 60 glutamic acid, and 24 of 65 aspartic acid residues).

MeSH Terms
Amino Acid Sequence Cyanogen Bromide Escherichia coli/enzymology Galactosidases Peptide Fragments/analysis beta-Galactosidase
Chemicals
Peptide Fragments Galactosidases beta-Galactosidase Cyanogen Bromide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fowler A V
Zabin I
Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
Published
1978-08-10
Pages
5505-9
Language
English
Region
United States
NLM ID
2985121R
Subset
IM
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