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PMID: 9736641 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Dynamic regulation of RGS2 suggests a novel mechanism in G-protein signaling and neuronal plasticity.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 18 ·No. 18 ·1998-09-15 ·Pages 7178-88

Ingi T, Krumins AM, Chidiac P, Brothers GM, Chung S, Snow BE, Barnes CA, Lanahan AA, Siderovski DP, Ross EM, Gilman AG, Worley PF

Abstract

Long-term neuronal plasticity is known to be dependent on rapid de novo synthesis of mRNA and protein, and recent studies provide insight into the molecules involved in this response. Here, we demonstrate that mRNA encoding a member of the regulator of G-protein signaling (RGS) family, RGS2, is rapidly induced in neurons of the hippocampus, cortex, and striatum in response to stimuli that evoke plasticity. Although several members of the RGS family are expressed in brain with discrete neuronal localizations, RGS2 appears unique in that its expression is dynamically responsive to neuronal activity. In biochemical assays, RGS2 stimulates the GTPase activity of the alpha subunit of Gq and Gi1. The effect on Gi1 was observed only after reconstitution of the protein in phospholipid vesicles containing M2 muscarinic acetylcholine receptors. RGS2 also inhibits both Gq- and Gi-dependent responses in transfected cells. These studies suggest a novel mechanism linking neuronal activity and signal transduction.

MeSH Terms
Animals COS Cells/chemistry,enzymology Calcium-Calmodulin-Dependent Protein Kinases/metabolism Cerebral Cortex/chemistry,cytology,enzymology Cocaine/pharmacology Dopamine Antagonists/pharmacology Dopamine Uptake Inhibitors/pharmacology Female GTP Phosphohydrolases/metabolism GTP-Binding Proteins/physiology Gene Expression/drug effects,physiology Genes, Immediate-Early/physiology Haloperidol/pharmacology Hippocampus/chemistry,cytology,enzymology Hydrolysis Lipid Metabolism Male Neuronal Plasticity/physiology Neurons/chemistry,drug effects,enzymology RNA, Messenger/metabolism Rats Rats, Inbred F344 Rats, Sprague-Dawley Receptors, Muscarinic/physiology Signal Transduction/drug effects,physiology Synaptic Transmission/drug effects,physiology
Chemicals
Dopamine Antagonists Dopamine Uptake Inhibitors RNA, Messenger Receptors, Muscarinic Calcium-Calmodulin-Dependent Protein Kinases GTP Phosphohydrolases GTP-Binding Proteins Cocaine Haloperidol
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Ingi T
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21210, USA.
Krumins A M
Chidiac P
Brothers G M
Chung S
Snow B E
Barnes C A
Lanahan A A
Siderovski D P
Ross E M
Gilman A G
Worley P F
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1998-09-15
Pages
7178-88
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6793237
Subset
IM
Grants
NIMH NIH HHS · MH53608 · United States
NIMH NIH HHS · K02 MH001152 · United States
NIGMS NIH HHS · R37 GM030355 · United States
NIMH NIH HHS · MH01152 · United States
NIA NIH HHS · R01 AG009219 · United States
NIMH NIH HHS · R01 MH053608 · United States
NIGMS NIH HHS · GM34497 · United States
NIGMS NIH HHS · R01 GM030355 · United States
NIGMS NIH HHS · R37 GM034497 · United States
NIGMS NIH HHS · R01 GM034497 · United States
External Links
PubMed source PMC Full Text View journal details
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