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PMID: 9744867 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Beadex encodes an LMO protein that regulates Apterous LIM-homeodomain activity in Drosophila wing development: a model for LMO oncogene function.

Genes & development ·Vol. 12 ·No. 18 ·1998-09-15 ·Pages 2912-20

Milán M, Diaz-Benjumea FJ, Cohen SM

Abstract

Formation of the dorsal-ventral axis of the Drosophila wing depends on activity of the LIM-homeodomain protein Apterous (Ap). Here we report that Ap activity levels are modulated by dLMO, the protein encoded by the Beadex (Bx) gene. Overexpression of dLMO in Bx mutants interferes with Apterous function. Conversely, Bx loss-of-function mutants fail to down-regulate Apterous activity at late stages of wing development. Biochemical analysis shows that dLMO protein competes for binding of Apterous to its cofactor Chip. These data suggest that Apterous activity depends on formation of a functional complex with Chip and that the relative levels of dLMO, Apterous, and Chip determine the level of Apterous activity. The dominant interference mechanism of dLMO action may serve as a model for the mechanism by which LMO oncogenes cause cancer when misexpressed in T cells.

MeSH Terms
Animals Base Sequence DNA Primers/genetics Drosophila/genetics,growth & development,physiology Drosophila Proteins Gene Expression Regulation, Developmental Genes, Homeobox Genes, Insect Homeodomain Proteins/genetics,physiology In Situ Hybridization Insect Proteins/physiology LIM-Homeodomain Proteins Macromolecular Substances Models, Biological Molecular Sequence Data Mutagenesis, Insertional Nuclear Proteins/physiology Oncogenes Phenotype Polymerase Chain Reaction Transcription Factors/physiology Wings, Animal/growth & development
Chemicals
Bx protein, Drosophila Chi protein, Drosophila DNA Primers Drosophila Proteins Homeodomain Proteins Insect Proteins LIM-Homeodomain Proteins Macromolecular Substances Nuclear Proteins Transcription Factors ap protein, Drosophila
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Milán M
European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany.
Diaz-Benjumea F J
Cohen S M
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.18.2912
Published
1998-09-15
Pages
2912-20
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC317163
Subset
IM
Databases
GENBANK
AJ010387, AJ010388, AJ010389
External Links
PubMed source DOI: 10.1101/gad.12.18.2912 PMC Full Text View journal details
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