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PMID: 9758823 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Characterization of genes involved in biosynthesis of a novel antibiotic from Burkholderia cepacia BC11 and their role in biological control of Rhizoctonia solani.

Applied and environmental microbiology ·Vol. 64 ·No. 10 ·1998-10-00 ·Pages 3939-47

Kang Y, Carlson R, Tharpe W, Schell MA

Abstract

Genetic manipulation of fluorescent pseudomonads has provided major insight into their production of antifungal molecules and their role in biological control of plant disease. Burkholderia cepacia also produces antifungal activities, but its biological control activity is much less well characterized, in part due to difficulties in applying genetic tools. Here we report genetic and biochemical characterization of a soil isolate of B. cepacia relating to its production of an unusual antibiotic that is very active against a variety of soil fungi. Purification and preliminary structural analyses suggest that this antibiotic (called AFC-BC11) is a novel lipopeptide associated largely with the cell membrane. Analysis of conditions for optimal production of AFC-BC11 indicated stringent environmental regulation of its synthesis. Furthermore, we show that production of AFC-BC11 is largely responsible for the ability of B. cepacia BC11 to effectively control the damping-off of cotton caused by the fungal pathogen Rhizoctonia solani in a gnotobiotic system. Using Tn5 mutagenesis, we identified, cloned, and characterized a region of the genome of strain BC11 that is required for production of this antifungal metabolite. DNA sequence analysis suggested that this region encodes proteins directly involved in the production of a nonribosomally synthesized lipopeptide.

MeSH Terms
Amino Acid Sequence Anti-Bacterial Agents/biosynthesis,chemistry,toxicity Antifungal Agents/biosynthesis Antimicrobial Cationic Peptides Base Sequence Biological Assay Burkholderia cepacia/genetics,isolation & purification,metabolism Cloning, Molecular DNA, Bacterial/chemistry,genetics Escherichia coli/genetics Gossypium/microbiology Molecular Sequence Data Peptides Pest Control, Biological Plant Diseases/microbiology Restriction Mapping Rhizoctonia/growth & development,pathogenicity Sequence Alignment Sequence Homology, Amino Acid Soil Microbiology
Chemicals
Anti-Bacterial Agents Antifungal Agents Antimicrobial Cationic Peptides DNA, Bacterial Peptides antibiotic AFC-BC11
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kang Y
Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA.
Carlson R
Tharpe W
Schell M A
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.64.10.3939-3947.1998
Published
1998-10-00
Pages
3939-47
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC106582
Subset
IM
Databases
GENBANK
AF076477
External Links
PubMed source DOI: 10.1128/AEM.64.10.3939-3947.1998 PMC Full Text View journal details
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