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PMID: 9765585 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Gram-negative bacteria produce membrane vesicles which are capable of killing other bacteria.

Journal of bacteriology ·Vol. 180 ·No. 20 ·1998-10-00 ·Pages 5478-83

Li Z, Clarke AJ, Beveridge TJ

Abstract

Naturally produced membrane vesicles (MVs), isolated from 15 strains of gram-negative bacteria (Citrobacter, Enterobacter, Escherichia, Klebsiella, Morganella, Proteus, Salmonella, and Shigella strains), lysed many gram-positive (including Mycobacterium) and gram-negative cultures. Peptidoglycan zymograms suggested that MVs contained peptidoglycan hydrolases, and electron microscopy revealed that the murein sacculi were digested, confirming a previous modus operandi (J. L. Kadurugamuwa and T. J. Beveridge, J. Bacteriol. 174:2767-2774, 1996). MV-sensitive bacteria possessed A1alpha, A4alpha, A1gamma, A2alpha, and A4gamma peptidoglycan chemotypes, whereas A3alpha, A3beta, A3gamma, A4beta, B1alpha, and B1beta chemotypes were not affected. Pseudomonas aeruginosa PAO1 vesicles possessed the most lytic activity.

MeSH Terms
Antibiosis Enterobacteriaceae/physiology,ultrastructure Gram-Negative Bacteria/drug effects Gram-Positive Bacteria/drug effects N-Acetylmuramoyl-L-alanine Amidase/metabolism,pharmacology Peptidoglycan/metabolism
Chemicals
Peptidoglycan N-Acetylmuramoyl-L-alanine Amidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Li Z
Canadian Bacterial Disease Network, Department of Microbiology, College of Biological Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
Clarke A J
Beveridge T J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.20.5478-5483.1998
Published
1998-10-00
Pages
5478-83
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC107602
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.180.20.5478-5483.1998 PMC Full Text View journal details
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