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PMID: 9774670 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Poly(A) tail length control in Saccharomyces cerevisiae occurs by message-specific deadenylation.

Molecular and cellular biology ·Vol. 18 ·No. 11 ·1998-11-00 ·Pages 6548-59

Brown CE, Sachs AB

Abstract

We report that newly synthesized mRNA poly(A) tails are matured to precise lengths by the Pab1p-dependent poly(A) nuclease (PAN) of Saccharomyces cerevisiae. These results provide evidence for an initial phase of mRNA deadenylation that is required for poly(A) tail length control. In RNA 3'-end processing extracts lacking PAN, transcripts are polyadenylated to lengths exceeding 200 nucleotides. By contrast, in extracts containing PAN, transcripts were produced with the expected wild-type poly(A) tail lengths of 60 to 80 nucleotides. The role for PAN in poly(A) tail length control in vivo was confirmed by the finding that mRNAs are produced with longer poly(A) tails in PAN-deficient yeast strains. Interestingly, wild-type yeast strains were found to produce transcripts which varied in their maximal poly(A) tail length, and this message-specific length control was lost in PAN-deficient strains. Our data support a model whereby mRNAs are polyadenylated by the 3'-end processing machinery with a long tail, possibly of default length, and then in a PAN-dependent manner, the poly(A) tails are rapidly matured to a message-specific length. The ability to control the length of the poly(A) tail for newly expressed mRNAs has the potential to be an important posttranscriptional regulatory step in gene expression.

MeSH Terms
Exoribonucleases/metabolism Fungal Proteins/metabolism Gene Expression Regulation, Fungal/genetics Microfilament Proteins Mutation/genetics Poly A/metabolism RNA Processing, Post-Transcriptional/physiology RNA, Messenger/metabolism Saccharomyces cerevisiae/physiology Saccharomyces cerevisiae Proteins
Chemicals
Fungal Proteins Microfilament Proteins PAN1 protein, S cerevisiae RNA, Messenger Saccharomyces cerevisiae Proteins Poly A Exoribonucleases PAN3 protein, S cerevisiae poly(A)-specific ribonuclease
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brown C E
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA.
Sachs A B
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.11.6548
Published
1998-11-00
Pages
6548-59
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC109240
Subset
IM
Grants
PHS HHS · 50308 · United States
External Links
PubMed source DOI: 10.1128/MCB.18.11.6548 PMC Full Text View journal details
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