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PMID: 9774677 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Glycogen synthase kinase 3beta and extracellular signal-regulated kinase inactivate heat shock transcription factor 1 by facilitating the disappearance of transcriptionally active granules after heat shock.

Molecular and cellular biology ·Vol. 18 ·No. 11 ·1998-11-00 ·Pages 6624-33

He B, Meng YH, Mivechi NF

Abstract

Heat shock transcription factor 1 (HSF-1) activates the transcription of heat shock genes in eukaryotes. Under normal physiological growth conditions, HSF-1 is a monomer. Its transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF-1 forms trimers, acquires DNA binding activity, increases transcriptional activity, and appears as punctate granules in the nucleus. In this study, using bromouridine incorporation and confocal laser microscopy, we demonstrated that newly synthesized pre-mRNAs colocalize to the HSF-1 punctate granules after heat shock, suggesting that these granules are sites of transcription. We further present evidence that glycogen synthase kinase 3beta (GSK-3beta) and extracellular signal-regulated kinase mitogen-activated protein kinase (ERK MAPK) participate in the down regulation of HSF-1 transcriptional activity. Transient increases in the expression of GSK-3beta facilitate the disappearance of HSF-1 punctate granules and reduce hsp-70 transcription after heat shock. We have also shown that ERK is the priming kinase for GSK-3beta. Taken together, these results indicate that GSK-3beta and ERK MAPK facilitate the inactivation of activated HSF-1 after heat shock by dispersing HSF-1 from the sites of transcription.

MeSH Terms
Calcium-Calmodulin-Dependent Protein Kinases/physiology DNA-Binding Proteins/metabolism Down-Regulation/physiology Fluorescent Antibody Technique Glycogen Synthase Kinase 3 Glycogen Synthase Kinases HSP70 Heat-Shock Proteins/metabolism HeLa Cells Heat Shock Transcription Factors Humans Microscopy, Fluorescence Nuclear Proteins/metabolism Phosphorylation Protein Conformation RNA Precursors/metabolism RNA, Messenger/metabolism Signal Transduction/physiology Temperature Transcription Factors Transcription, Genetic/genetics Transfection/genetics
Chemicals
DNA-Binding Proteins HSF1 protein, human HSP70 Heat-Shock Proteins Heat Shock Transcription Factors Nuclear Proteins RNA Precursors RNA, Messenger Transcription Factors Glycogen Synthase Kinases Calcium-Calmodulin-Dependent Protein Kinases Glycogen Synthase Kinase 3
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
He B
Institute of Molecular Medicine and Genetics, Department of Radiology, Medical College of Georgia, Augusta, Georgia 30912, USA.
Meng Y H
Mivechi N F
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.11.6624
Published
1998-11-00
Pages
6624-33
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC109247
Subset
IM
Grants
NCI NIH HHS · CA62130 · United States
External Links
PubMed source DOI: 10.1128/MCB.18.11.6624 PMC Full Text View journal details
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