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PMID: 977652 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cytochalasin B inhibits actin-related gelation of HeLa cell extracts.

The Journal of cell biology ·Vol. 71 ·No. 1 ·1976-10-00 ·Pages 303-7

Weihing RR

Abstract

When the 100,000 g supernatant fraction (extract) of HeLa cells lysed in a buffer containing sucrose, ATP, DTE, EGTA, imidazole, and Triton X-100 is incubated at 25 degrees C, it gels, and actin and a HMWP are progressively enriched in the extract and in gel isolated from extract. CB (greater than or equal to 0.25 muM) inhibits gelation and specifically lowers the concentrations of actin and the HMWP in the fraction which sediments at 100,000 g after incubation. These results indicate that actin and HMWP are partly disaggregated by cytochalasin treatment, and thus that their aggregation is related gelation. Inasmuch as previous results showed that actin is present and HMWP is enriched in the plasma membrane fraction of HeLa cells, the results also point to a possible relation between plasma membrane-associated gel and in vivo effects of CB.

MeSH Terms
Actins/metabolism Contractile Proteins/metabolism Cytochalasin B/pharmacology Dimethyl Sulfoxide/pharmacology Dose-Response Relationship, Drug HeLa Cells
Chemicals
Actins Contractile Proteins Cytochalasin B Dimethyl Sulfoxide
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Weihing R R
References (34)
34 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.71.1.303
Published
1976-10-00
Pages
303-7
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109735
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.71.1.303 PMC Full Text View journal details
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