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PMID: 9789088 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Gene discovery in the wood-forming tissues of poplar: analysis of 5, 692 expressed sequence tags.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 22 ·1998-10-27 ·Pages 13330-5

Sterky F, Regan S, Karlsson J, Hertzberg M, Rohde A, Holmberg A, Amini B, Bhalerao R, Larsson M, Villarroel R, Van Montagu M, Sandberg G, Olsson O, Teeri TT, Boerjan W, Gustafsson P, Uhlén M, Sundberg B, Lundeberg J

Abstract

A rapidly growing area of genome research is the generation of expressed sequence tags (ESTs) in which large numbers of randomly selected cDNA clones are partially sequenced. The collection of ESTs reflects the level and complexity of gene expression in the sampled tissue. To date, the majority of plant ESTs are from nonwoody plants such as Arabidopsis, Brassica, maize, and rice. Here, we present a large-scale production of ESTs from the wood-forming tissues of two poplars, Populus tremula L. x tremuloides Michx. and Populus trichocarpa 'Trichobel.' The 5,692 ESTs analyzed represented a total of 3,719 unique transcripts for the two cDNA libraries. Putative functions could be assigned to 2,245 of these transcripts that corresponded to 820 protein functions. Of specific interest to forest biotechnology are the 4% of ESTs involved in various processes of cell wall formation, such as lignin and cellulose synthesis, 5% similar to developmental regulators and members of known signal transduction pathways, and 2% involved in hormone biosynthesis. An additional 12% of the ESTs showed no significant similarity to any other DNA or protein sequences in existing databases. The absence of these sequences from public databases may indicate a specific role for these proteins in wood formation. The cDNA libraries and the accompanying database are valuable resources for forest research directed toward understanding the genetic control of wood formation and future endeavors to modify wood and fiber properties for industrial use.

MeSH Terms

Arabidopsis/genetics Brassica/genetics Cloning, Molecular Crosses, Genetic DNA, Complementary Enzymes/genetics Expressed Sequence Tags Gene Expression Gene Library Genes, Plant Molecular Sequence Data Oryza/genetics Plant Proteins/genetics Trees/anatomy & histology,cytology,genetics Zea mays/genetics

Chemicals

DNA, Complementary Enzymes Plant Proteins

Authors & Affiliations

19 authors, click to expand affiliations / ORCID

Sterky F

Department of Biotechnology, Kungl Tekniska Högskolan, Royal Institute of Technology, SE-10044 Stockholm, Sweden.

Regan S

Karlsson J

Hertzberg M

Rohde A

Holmberg A

Amini B

Bhalerao R

Larsson M

Villarroel R

Van Montagu M

Sandberg G

Olsson O

Teeri T T

Boerjan W

Gustafsson P

Uhlén M

Sundberg B

Lundeberg J

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.95.22.13330

Published

1998-10-27

Pages

13330-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC23802

Subset

Databases

GENBANK

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PubMed source DOI: 10.1073/pnas.95.22.13330 PMC Full Text View journal details

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Gene discovery in the wood-forming tissues of poplar: analysis of 5, 692 expressed sequence tags.

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