Binding of hepatitis C virus to CD81.

Science (New York, N.Y.) ·Vol. 282 ·No. 5390 ·1998-10-30 ·Pages 938-41

Pileri P, Uematsu Y, Campagnoli S, Galli G, Falugi F, Petracca R, Weiner AJ, Houghton M, Rosa D, Grandi G, Abrignani S

Abstract

Chronic hepatitis C virus (HCV) infection occurs in about 3 percent of the world's population and is a major cause of liver disease. HCV infection is also associated with cryoglobulinemia, a B lymphocyte proliferative disorder. Virus tropism is controversial, and the mechanisms of cell entry remain unknown. The HCV envelope protein E2 binds human CD81, a tetraspanin expressed on various cell types including hepatocytes and B lymphocytes. Binding of E2 was mapped to the major extracellular loop of CD81. Recombinant molecules containing this loop bound HCV and antibodies that neutralize HCV infection in vivo inhibited virus binding to CD81 in vitro.

MeSH Terms

Amino Acid Sequence Animals Antibodies/immunology Antibodies, Viral/immunology Antigens, CD/chemistry,genetics,immunology,metabolism Cell Line DNA, Complementary Gene Library Hepacivirus/immunology,metabolism Hepatitis C/immunology Humans Liver/cytology,immunology,virology Lymphocytes/immunology,virology Membrane Proteins/chemistry,genetics,immunology,metabolism Mice Molecular Sequence Data Pan troglodytes Recombinant Fusion Proteins/metabolism Recombinant Proteins/metabolism,pharmacology Sequence Alignment Tetraspanin 28 Tumor Cells, Cultured Viral Envelope Proteins/genetics,immunology,metabolism

Chemicals

Antibodies Antibodies, Viral Antigens, CD CD81 protein, human Cd81 protein, mouse DNA, Complementary Membrane Proteins Recombinant Fusion Proteins Recombinant Proteins Tetraspanin 28 Viral Envelope Proteins glycoprotein E2, Hepatitis C virus

Authors & Affiliations

11 authors, click to expand affiliations / ORCID

Pileri P

IRIS, Chiron, Siena 53100, Italy.

Uematsu Y

Campagnoli S

Galli G

Falugi F

Petracca R

Weiner A J

Houghton M

Rosa D

Grandi G

Abrignani S

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.282.5390.938

Published

1998-10-30

Pages

938-41

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.282.5390.938 View journal details

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Binding of hepatitis C virus to CD81.

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