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PMID: 9808745 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The heat-shock element is a functional component of the Arabidopsis APX1 gene promoter.

Plant physiology ·Vol. 118 ·No. 3 ·1998-11-00 ·Pages 1005-14

Storozhenko S, De Pauw P, Van Montagu M, Inzé D, Kushnir S

Abstract

Ascorbate peroxidases are important enzymes that detoxify hydrogen peroxide within the cytosol and chloroplasts of plant cells. To better understand their role in oxidative stress tolerance, the transcriptional regulation of the apx1 gene from Arabidopsis was studied. The apx1 gene was expressed in all tested organs of Arabidopsis; mRNA levels were low in roots, leaves, and stems and high in flowers. Steady-state mRNA levels in leaves or cell suspensions increased after treatment with methyl viologen, ethephon, high temperature, and illumination of etiolated seedlings. A putative heat-shock cis element found in the apx1 promoter was shown to be recognized by the tomato (Lycopersicon esculentum) heat-shock factor in vitro and to be responsible for the in vivo heat-shock induction of the gene. The heat-shock cis element also contributed partially to the induction of the gene by oxidative stress. By using in vivo dimethyl sulfate footprinting, we showed that proteins interacted with a G/C-rich element found in the apx1 promoter.

MeSH Terms
Arabidopsis/enzymology,genetics Ascorbate Peroxidases Base Sequence DNA Footprinting DNA Primers DNA, Complementary Heat-Shock Proteins/metabolism Oxidative Stress Peroxidases/genetics Promoter Regions, Genetic RNA, Messenger/genetics,metabolism Sequence Homology, Nucleic Acid
Chemicals
DNA Primers DNA, Complementary Heat-Shock Proteins RNA, Messenger Peroxidases Ascorbate Peroxidases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Storozhenko S
Laboratorium voor Genetica, Departement Genetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Belgium.
De Pauw P
Van Montagu M
Inzé D
Kushnir S
References (47)
47 references, click to expand
  1. Mouse heat shock transcription factors 1 and 2 prefer a trimeric binding site but interact differently with the HSP70 heat shock element.
    Mol Cell Biol. 1993 Jun;13(6):3370-83 PMID: 8497256
  2. Expression of Arabidopsis cytosolic ascorbate peroxidase gene in response to ozone or sulfur dioxide.
    Plant Mol Biol. 1995 Nov;29(3):479-89 PMID: 8534847
  3. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves.
    Plant Physiol. 1992 Apr;98(4):1222-7 PMID: 16668779
  4. Control of the appearance of ascorbate peroxidase (EC 1.11.1.11) in mustard seedling cotyledons by phytochrome and photooxidative treatments.
    Planta. 1992 Mar;186(4):600-8 PMID: 24186792
  5. Genomic DNA structure of a gene encoding cytosolic ascorbate peroxidase from Arabidopsis thaliana.
    FEBS Lett. 1993 Jan 11;315(3):313-7 PMID: 8422923
  6. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  7. Protein traffic on the heat shock promoter: parking, stalling, and trucking along.
    Cell. 1993 Jul 16;74(1):1-4 PMID: 8334697
  8. Oxidative stress induced heat shock factor phosphorylation and HSF-dependent activation of yeast metallothionein gene transcription.
    Genes Dev. 1996 Mar 1;10(5):592-603 PMID: 8598289
  9. Protective systems against active oxygen species in spinach: response to cold acclimation in excess light.
    Planta. 1990 Feb;180(3):383-9 PMID: 24202017
  10. The anaerobic responsive element contains two GC-rich sequences essential for binding a nuclear protein and hypoxic activation of the maize Adh1 promoter.
    Nucleic Acids Res. 1991 Dec;19(25):7053-60 PMID: 1766868
  11. OCSBF-1, a maize ocs enhancer binding factor: isolation and expression during development.
    Plant Cell. 1990 Sep;2(9):891-903 PMID: 2152133
  12. Functional analysis of DNA sequences responsible for ethylene regulation of a bean chitinase gene in transgenic tobacco.
    Plant Cell. 1989 Jun;1(6):599-607 PMID: 2535512
  13. The Hsf world: classification and properties of plant heat stress transcription factors.
    Cell Stress Chaperones. 1996 Dec;1(4):215-23 PMID: 9222607
  14. Mutational analysis of a plant heat shock element.
    Plant Mol Biol. 1992 Jul;19(4):665-75 PMID: 1627779
  15. Molecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidase.
    J Biol Chem. 1992 Oct 25;267(30):21802-7 PMID: 1400489
  16. Hydrogen peroxide (H2O2) induces actin and some heat-shock proteins in Drosophila cells.
    Eur J Biochem. 1988 Jan 15;171(1-2):163-70 PMID: 3123230
  17. Iron triggers a rapid induction of ascorbate peroxidase gene expression in Brassica napus.
    FEBS Lett. 1997 Jun 30;410(2-3):195-200 PMID: 9237628
  18. Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress.
    Plant Cell. 1997 Apr;9(4):627-40 PMID: 9144965
  19. Ligation-anchored PCR: a simple amplification technique with single-sided specificity.
    Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9823-5 PMID: 1409706
  20. The Cauliflower Mosaic Virus 35S Promoter: Combinatorial Regulation of Transcription in Plants.
    Science. 1990 Nov 16;250(4983):959-66 PMID: 17746920
  21. Yeast heat shock transcription factor contains a flexible linker between the DNA-binding and trimerization domains. Implications for DNA binding by trimeric proteins.
    J Biol Chem. 1994 Apr 29;269(17):12475-81 PMID: 8175654
  22. Evidence for the Presence of the Ascorbate-Glutathione Cycle in Mitochondria and Peroxisomes of Pea Leaves.
    Plant Physiol. 1997 May;114(1):275-284 PMID: 12223704
  23. Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought.
    Plant J. 1994 Mar;5(3):397-405 PMID: 8180623
  24. Stable binding of Drosophila heat shock factor to head-to-head and tail-to-tail repeats of a conserved 5 bp recognition unit.
    Cell. 1989 Dec 1;59(5):797-806 PMID: 2590940
  25. A flower-specific Myb protein activates transcription of phenylpropanoid biosynthetic genes.
    EMBO J. 1994 Jan 1;13(1):128-37 PMID: 8306956
  26. Photoregulated gene expression may involve ubiquitous DNA binding proteins.
    EMBO J. 1990 Nov;9(11):3415-27 PMID: 2209551
  27. Induction of ascorbate peroxidase and glutathione reductase activities by interactions of mixtures of air pollutants.
    Free Radic Res Commun. 1987;3(1-5):193-7 PMID: 3508431
  28. Genomic sequencing and methylation analysis by ligation mediated PCR.
    Science. 1989 Nov 10;246(4931):810-3 PMID: 2814502
  29. Mapping transcription start points on cloned genomic DNA with T4 DNA polymerase: a precise and convenient technique.
    Gene. 1986;42(1):21-9 PMID: 3721201
  30. Three tomato genes code for heat stress transcription factors with a region of remarkable homology to the DNA-binding domain of the yeast HSF.
    EMBO J. 1990 Dec;9(13):4495-501 PMID: 2148291
  31. Cytosolic ascorbate peroxidase from Arabidopsis thaliana L. is encoded by a small multigene family.
    Planta. 1996;198(1):64-9 PMID: 8580771
  32. Strong cellular preference in the expression of a housekeeping gene of Arabidopsis thaliana encoding S-adenosylmethionine synthetase.
    Plant Cell. 1989 Jan;1(1):81-93 PMID: 2535470
  33. Ultraviolet-B- and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana.
    Plant Physiol. 1996 Jan;110(1):125-36 PMID: 8587977
  34. Cloning and sequencing of a cDNA encoding ascorbate peroxidase from Arabidopsis thaliana.
    Plant Mol Biol. 1992 Feb;18(4):691-701 PMID: 1558944
  35. Heat shock transcription factors: structure and regulation.
    Annu Rev Cell Dev Biol. 1995;11:441-69 PMID: 8689565
  36. Fine structure analyses of the Drosophila and Saccharomyces heat shock factor--heat shock element interactions.
    Nucleic Acids Res. 1994 Jan 25;22(2):167-73 PMID: 8121800
  37. Engineering herbicide resistance in plants by expression of a detoxifying enzyme.
    EMBO J. 1987 Sep;6(9):2513-8 PMID: 16453789
  38. An efficient method for isolation of RNA from tissue cultured plant cells.
    Nucleic Acids Res. 1991 Nov 11;19(21):6055 PMID: 1719489
  39. Differential accumulation of antioxidant mRNAs in Arabidopsis thaliana exposed to ozone.
    Plant Physiol. 1995 Sep;109(1):203-12 PMID: 7480322
  40. In vivo footprinting of a muscle specific enhancer by ligation mediated PCR.
    Science. 1989 Nov 10;246(4931):780-6 PMID: 2814500
  41. Proteins containing non-native disulfide bonds generated by oxidative stress can act as signals for the induction of the heat shock response.
    J Cell Physiol. 1997 May;171(2):143-51 PMID: 9130461
  42. From sequence analysis of three novel ascorbate peroxidases from Arabidopsis thaliana to structure, function and evolution of seven types of ascorbate peroxidase.
    Biochem J. 1997 Sep 1;326 ( Pt 2):305-10 PMID: 9291097
  43. Differential transcript induction of parsley pathogenesis-related proteins and of a small heat shock protein by ozone and heat shock.
    Plant Mol Biol. 1997 Jan;33(2):343-50 PMID: 9037151
  44. Oxidative stress is involved in heat-induced cell death in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1996 May 14;93(10):5116-21 PMID: 8643537
  45. Isolation and characterization of a cellulase gene family member expressed during avocado fruit ripening.
    Mol Gen Genet. 1990 Aug;223(1):76-86 PMID: 2259345
  46. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection.
    Proc Natl Acad Sci U S A. 1988 Aug;85(15):5536-40 PMID: 16593964
  47. Hydrogen peroxide-inducible proteins in Salmonella typhimurium overlap with heat shock and other stress proteins.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8059-63 PMID: 3534881
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.118.3.1005
Published
1998-11-00
Pages
1005-14
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC34773
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.118.3.1005 PMC Full Text View journal details
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