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PMID: 9811772 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Construction and characterization of hexon-chimeric adenoviruses: specification of adenovirus serotype.

Journal of virology ·Vol. 72 ·No. 12 ·1998-12-00 ·Pages 10260-4

Gall JG, Crystal RG, Falck-Pedersen E

Abstract

This study has used the strategy of gene replacement to characterize the contribution of the adenovirus (Ad) capsid protein hexon to serotype definition. By replacing the Ad type 5 (Ad5) hexon gene with sequences from Ad2, we have changed the type specificity of the chimeric virus. The type-determining epitopes are primarily associated with loop 1 of hexon and, to a much lesser degree, with loop 2. In spite of the serotype distinctiveness of the chimeric hexon viruses, epitope similarity between the vectors resulted in a low level of cross-reactive neutralizing antibody, which in combination with activated cellular and innate arms of the immune system is sufficient to suppress gene transduction following readministration in vivo.

MeSH Terms
Adenoviridae Infections/immunology,prevention & control,virology Animals Antibodies, Viral Antigens, Viral/genetics Capsid/genetics,immunology Capsid Proteins Cell Line Chimera/genetics,immunology Cross Reactions Epitopes/genetics Genes, Viral Genetic Vectors Humans Mastadenovirus/classification,genetics,immunology Neutralization Tests Rats Rats, Sprague-Dawley Recombination, Genetic Serotyping Transduction, Genetic
Chemicals
Antibodies, Viral Antigens, Viral Capsid Proteins Epitopes hexon capsid protein, Adenovirus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gall J G
Department of Microbiology, W. R. Hearst Research Foundation, Cornell University Medical College, New York, New York 10021, USA.
Crystal R G
Falck-Pedersen E
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.72.12.10260-10264.1998
Published
1998-12-00
Pages
10260-4
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC110610
Subset
IM
Grants
NHLBI NIH HHS · P01 HL051746 · United States
NHLBI NIH HHS · P01 HL51746 · United States
External Links
PubMed source DOI: 10.1128/JVI.72.12.10260-10264.1998 PMC Full Text View journal details
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